Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human ISL LIM Homeobox Protein 1 Polyclonal Antibody | anti-ISL1 antibody
ISL LIM Homeobox Protein 1 (ISL1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]
Uniprot Description
ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer.
Protein type: Motility/polarity/chemotaxis; DNA-binding; Transcription factor; Cell development/differentiation; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 5q11.1
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: bHLH transcription factor binding; chromatin binding; estrogen receptor binding; ligand-dependent nuclear receptor binding; protein binding; zinc ion binding
Biological Process: axon regeneration; mesenchymal cell differentiation; negative regulation of estrogen receptor signaling pathway; negative regulation of inflammatory response; negative regulation of neuron apoptosis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; neuron fate specification; pancreas development; peripheral nervous system neuron axonogenesis; pharyngeal system development; pituitary gland development; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of histone acetylation; positive regulation of insulin secretion; positive regulation of interferon-gamma production; positive regulation of interleukin-1 alpha production; positive regulation of interleukin-1 beta production; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of Stat3 protein; retinal ganglion cell axon guidance; sensory system development; spinal cord motor neuron cell fate specification; spinal cord motor neuron differentiation; transcription from RNA polymerase II promoter; trigeminal nerve development; ventricular cardiac muscle morphogenesis; visceral motor neuron differentiation
Research Articles on ISL1
Similar Products
Product Notes
The ISL1 isl1 (Catalog #AAA2032369) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ISL LIM Homeobox Protein 1 (ISL1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ISL LIM Homeobox Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ISL1 isl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ISL LIM Homeobox Protein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
