Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with IRS2 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).IRS2 immunoreactivity is localized to Cytoplasm significantly. )
Rabbit anti-Human IRS-2 Polyclonal Antibody | anti-IRS-2 antibody
IRS-2, CT (Insulin Receptor Substrate 2, IRS2) (APC)
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with IRS2 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).IRS2 immunoreactivity is localized to Cytoplasm significantly. )
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with IRS2 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).IRS2 immunoreactivity is localized to Cytoplasm significantly. )
Western Blot (WB)
(Western blot analysis of anti-IRS2 Antibody (C-term) in 293 cell line lysates (35ug/lane). IRS2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-IRS2 Antibody (C-term) in 293 cell line lysates (35ug/lane). IRS2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]
Uniprot Description
IRS2: May mediate the control of various cellular processes by insulin. Interacts with PHIP.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: plasma membrane; cytosol
Molecular Function: protein domain specific binding; signal transducer activity; protein binding; phosphoinositide 3-kinase binding; protein kinase binding; protein phosphatase binding; insulin receptor binding
Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of kinase activity; regulation of lipid metabolic process; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; lipid homeostasis; positive regulation of insulin secretion; glucose metabolic process; signal transduction; positive regulation of fatty acid beta-oxidation; positive regulation of glucose import; cell proliferation; mammary gland development; cellular response to insulin stimulus; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of B cell proliferation; response to glucose stimulus; insulin receptor signaling pathway; innate immune response; brain development; negative regulation of B cell apoptosis; positive regulation of cell migration
Disease: Diabetes Mellitus, Noninsulin-dependent
Research Articles on IRS-2
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Product Notes
The IRS-2 irs2 (Catalog #AAA6483602) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IRS-2, CT (Insulin Receptor Substrate 2, IRS2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IRS-2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IRS-2 irs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRS-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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