Rabbit anti-Human IRGM Polyclonal Antibody | anti-IRGM antibody

IRGM, CT (IRGM, IFI1, IRGM1, LRG47, Immunity-related GTPase family M protein, Immunity-related GTPase family M protein 1, Interferon-inducible protein 1, LPS-stimulated RAW 264.7 macrophage protein 47 homolog) (AP)

Cat. Num. AAA6310949

• Gene Names: IRGM; IFI1; IRGM1; LRG47; LRG-47
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IRGM; Polyclonal Antibody; CT (IRGM; IFI1; IRGM1; LRG47; Immunity-related GTPase family M protein; Immunity-related GTPase family M protein 1; Interferon-inducible protein 1; LPS-stimulated RAW 264.7 macrophage protein 47 homolog) (AP); LPS-stimulated RAW 264.7 macrophage protein 47 homolog; anti-IRGM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-IRGM antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IRGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the C-terminal region of human IRGM.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(IRGM Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the IRGM antibody detected the IRGM protein (arrow).)

Immunohistochemistry (IHC)

(IRGM Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IRGM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-IRGM antibody

This gene encodes a member of the p47 immunity-related GTPase family. The encoded protein may play a role in the innate immune response by regulating autophagy formation in response to intracellular pathogens. Polymorphisms that affect the normal expression of this gene are associated with a susceptibility to Crohn's disease and tuberculosis.

Product Categories/Family for anti-IRGM antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Che, N., et al. Clin. Chim. Acta 411 (21-22), 1645-1649 (2010): Wolfkamp, S.C., et al. Eur J Gastroenterol Hepatol 22(8):933-937(2010). Latiano, A., et al. Inflamm. Bowel Dis. 16(7):1108-1117(2010). Prescott, N.J., et al. Hum. Mol. Genet. 19(9):1828-1839(2010). Bekpen, C., et al. PLoS Genet. 5 (3), E1000403 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 224809576
• NCBI GeneID: 345611
• NCBI Accession #: NP_001139277.1
• NCBI GenBank Nucleotide #: NM_001145805.1
• UniProt Accession #: A1A4Y4; B3VEX0
• Molecular Weight: 20,142 Da
• NCBI Official Full Name: immunity-related GTPase family M protein
• NCBI Official Synonym Full Names: immunity-related GTPase family, M
• NCBI Official Symbol: IRGM
• NCBI Official Synonym Symbols: IFI1; IRGM1; LRG47; LRG-47
• NCBI Protein Information: immunity-related GTPase family M protein; LPS-stimulated RAW 264.7 macrophage protein 47 homolog; LRG-47-like protein; immunity-related GTPase family, M1; interferon-inducible protein 1
• UniProt Protein Name: Immunity-related GTPase family M protein
• Protein Family: Immunity-related GTPase family M protein
• UniProt Gene Name: IRGM
• UniProt Synonym Gene Names: IFI1; IRGM1; LRG47; LRG-47
• UniProt Entry Name: IRGM_HUMAN

NCBI Description

This gene encodes a member of the p47 immunity-related GTPase family. The encoded protein may play a role in the innate immune response by regulating autophagy formation in response to intracellular pathogens. Polymorphisms that affect the normal expression of this gene are associated with a susceptibility to Crohn's disease and tuberculosis.[provided by RefSeq, Oct 2010]

Uniprot Description

IRGM: Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility. Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19). A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Belongs to the interferon-inducible GTPase family.

Protein type: Hydrolase; Endoplasmic reticulum; EC 3.6.5.-

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell projection; phagocytic cup

Molecular Function: GTP binding; hydrolase activity

Biological Process: autophagy; innate immune response; inflammatory response

Disease: Mycobacterium Tuberculosis, Susceptibility To; Inflammatory Bowel Disease 19