Rabbit anti-Human IRF3 Polyclonal Antibody | anti-IRF3 antibody

Anti-IRF3 Antibody

Cat. Num. AAA4150026

• Gene Names: IRF3; IIAE7
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: IRF3; Polyclonal Antibody; Anti-IRF3 Antibody; anti-IRF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of brain cell lines, using IRF3 antibody. Immunohistochemistry of paraffin-embedded human kidney using IRF3 antibody at dilution of 1:200 (400x lens). Immunohistochemistry of paraffin-embedded human lung using IRF3 antibody at dilution of 1:200 (400x lens). Immunofluorescence analysis of U2OS cell using IRF3 antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 281

• Applicable Applications for anti-IRF3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:50- 1:200
• Immunogen: Recombinant protein of human IRF3
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Related Product Information for anti-IRF3 antibody

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF33/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal Ser and Thr residues (between 385 and 405), leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity (5). During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and a kinase complex that includes IKKug and TBK1 (6, 7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double-stranded RNA treatment. These events likely play a role in activation of IRF-3 (8).

NCBI and Uniprot Product Information

• NCBI GI #: 308199454
• NCBI GeneID: 3661
• NCBI Accession #: NP_001184054.1
• NCBI GenBank Nucleotide #: NM_001197125.1
• UniProt Accession #: Q14653; Q5FBY1; Q5FBY2; Q5FBY4; Q7Z5G6; A8K7L2; B2RAZ3
• Molecular Weight: 12,330 Da
• NCBI Official Full Name: interferon regulatory factor 3 isoform 5
• NCBI Official Synonym Full Names: interferon regulatory factor 3
• NCBI Official Symbol: IRF3
• NCBI Official Synonym Symbols: IIAE7
• NCBI Protein Information: interferon regulatory factor 3
• UniProt Protein Name: Interferon regulatory factor 3
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF3
• UniProt Synonym Gene Names: IRF-3

NCBI Description

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; transcription cofactor activity

Biological Process: apoptosis; defense response to virus; negative regulation of interferon type I production; positive regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; response to DNA damage stimulus; transcription from RNA polymerase II promoter

Disease: Herpes Simplex Encephalitis, Susceptibility To, 7