Rabbit anti-Human, Mouse IQCJ Polyclonal Antibody | anti-IQCJ antibody

IQCJ, CT (IQCJ, IQ domain-containing protein J) (FITC)

Cat. Num. AAA6310776

• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IQCJ; Polyclonal Antibody; CT (IQCJ; IQ domain-containing protein J) (FITC); IQ domain-containing protein J; anti-IQCJ antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-IQCJ antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IQCJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-130 amino acids from the C-terminal region of human IQCJ.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IQCJ Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the IQCJ antibody detected the IQCJ protein (arrow).)

Western Blot (WB)

(IQCJ Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the IQCJ antibody detected the IQCJ protein (arrow).)

Immunohistochemistry (IHC)

(IQCJ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IQCJ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(IQCJ Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-IQCJ antibody

Antibodies; Proteins

References

Martin, P.M., et al. J. Neurosci. 28(24):6111-6117(2008). Kwasnicka-Crawford, D.A., et al. Biochem. Biophys. Res. Commun. 350(4):890-899(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 111607465
• NCBI GeneID: 654502
• NCBI Accession #: NP_001036170.1
• NCBI GenBank Nucleotide #: NM_001042705.2
• UniProt Accession #: Q1A5X6; Q1A5X5; B7ZMM2; B9EH97
• Molecular Weight: 18,226 Da
• NCBI Official Full Name: IQ domain-containing protein J isoform CaMBPv1
• NCBI Official Synonym Full Names: IQ motif containing J
• NCBI Official Symbol: IQCJ
• NCBI Protein Information: IQ domain-containing protein J; calmodulin binding protein
• UniProt Protein Name: IQ domain-containing protein J
• Protein Family: IQ domain-containing protein
• UniProt Gene Name: IQCJ
• UniProt Entry Name: IQCJ_HUMAN

Uniprot Description

IQCJ: 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q25.32