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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human skin tissue slide using MBS7600601 (Involucrin Antibody) at dilution of 1:50)

Rabbit anti-Human, Mouse Involucrin Polyclonal Antibody | anti-IVL antibody

Involucrin Rabbit Polyclonal

Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purification: Immunogen affinity purified
Purity: > = 95% as determined by SDS-PAGE
Synonyms
Involucrin; Polyclonal Antibody; Involucrin Rabbit Polyclonal; anti-IVL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Other species are not tested. Please decide the specificity by homology.
Purity/Purification
Purification: Immunogen affinity purified
Purity: > = 95% as determined by SDS-PAGE
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Sequence Length
585
Applicable Applications for anti-IVL antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:500
Immunogen
Involucrin
Preparation and Storage
Store at -20 degree C for 24 months (Avoid repeated freeze/thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skin tissue slide using MBS7600601 (Involucrin Antibody) at dilution of 1:50)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human skin tissue slide using MBS7600601 (Involucrin Antibody) at dilution of 1:50)

Western Blot (WB)

(Raji cells were subjected to SDS PAGE followed by western blot with MBS7600601 (IVL antibody) at dilution of 1:500)

Western Blot (WB) (Raji cells were subjected to SDS PAGE followed by western blot with MBS7600601 (IVL antibody) at dilution of 1:500)
Related Product Information for anti-IVL antibody
N/AInvolucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, while different reports provided variable results ranging from 90-140 kDa.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80 kDa
NCBI Official Full Name
involucrin
NCBI Official Synonym Full Names
involucrin
NCBI Official Symbol
IVL
NCBI Protein Information
involucrin
UniProt Protein Name
Involucrin
Protein Family
UniProt Gene Name
IVL
UniProt Entry Name
INVO_HUMAN

NCBI Description

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

Uniprot Description

IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cornified envelope; cytoplasm

Molecular Function: protein binding; protein binding, bridging; structural molecule activity

Biological Process: isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; keratinocyte differentiation; peptide cross-linking; response to UV-B

Research Articles on IVL

Similar Products

Product Notes

The IVL ivl (Catalog #AAA7600601) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Involucrin Rabbit Polyclonal reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Involucrin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:500. Researchers should empirically determine the suitability of the IVL ivl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Involucrin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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