IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF antibody.)
Mouse BRAF Monoclonal Antibody | anti-BRAF antibody
BRAF antibody
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
BRAF, Antibody; BRAF antibody; Mouse monoclonal BRAF antibody; Monoclonal BRAF antibody; Anti-BRAF antibody; V-raf murine sarcoma viral oncogene homolog B1 antibody; BRAF1 antibody; RAFB1 antibody; B-RAF1 antibody; B RAF1 antibody; anti-BRAF antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1H12
Form/Format
Liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
271
Applicable Applications for anti-BRAF antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA
Immunogen
BRAF antibody was raised in Mouse using a purified recombinant fragment of human BRAF expressed in E Coli as the immunogen.
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF antibody.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF antibody.)
WB (Western Blot)
(Western Blot showing BRAF antibody used against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.)
WB (Western Blot)
(Western Blot showing BRAF antibody used against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye)
Related Product Information for anti-BRAF antibody
BRAF is one of the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylation sites. B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region, the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras.
Product Categories/Family for anti-BRAF antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
30,192 Da
NCBI Official Full Name
BraF
UniProt Protein Name
BraF
UniProt Gene Name
braF
Similar Products
Product Notes
The BRAF braf (Catalog #AAA225010) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BRAF can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the BRAF braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRAF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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