Western Blot (WB)
(Western blot analysis of human interleukin-17B using recombinant human interleukin-17B diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Human interleukin-17B antibody (MBS226170) followed by a compatible secondary antibody.)
Rabbit anti-Human INTERLEUKIN-17B Polyclonal Antibody | anti-IL-17B antibody
Rabbit anti Human INTERLEUKIN-17B:Preservative Free
Gene Names
IL17B; NIRF; IL-20; IL-17B; ZCYTO7
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Produced from sera of rabbits immunized with recombinant human interleukin-17B and purified by affinity chromatography
Synonyms
INTERLEUKIN-17B; Polyclonal Antibody; Rabbit anti Human INTERLEUKIN-17B:Preservative Free; IL-17B antibody; anti-IL-17B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Produced from sera of rabbits immunized with recombinant human interleukin-17B and purified by affinity chromatography
Form/Format
Phosphate buffered saline
Purified IgG - lyophilized
Purified IgG - lyophilized
Concentration
IgG concentration 1.0mg/ml after reconstitution (varies by lot)
Applicable Applications for anti-IL-17B antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 0.5ug/ml to 2.0ug/ml
WB: 0.1ug/ml to 0.2ug/ml
WB: 0.1ug/ml to 0.2ug/ml
Immunogen
E Coli derived recombinant human interleukin-17B
Endotoxin Level
<0.1ng/ug of protein (<1EU/ug)
Conjugation
Preservative Free
Reconstitution
Reconstitute with 0.1ml distilled water
Preparation and Storage
Prior to reconstitution store at -20 degree C.
After reconstitution store at -20 degree C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20 degree C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis of human interleukin-17B using recombinant human interleukin-17B diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Human interleukin-17B antibody (MBS226170) followed by a compatible secondary antibody.)
Western Blot (WB)
(Western blot analysis of human interleukin-17B using recombinant human interleukin-17B diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Human interleukin-17B antibody (MBS226170) followed by a compatible secondary antibody.)
Related Product Information for anti-IL-17B antibody
Rabbit anti Human interleukin-17B antibody recognizes interleukin-17B (IL-17B) also known as cytokine Zcyto7, IL-20 and neuronal interleukin-17-related factor.
IL17B is a member of the IL-17 superfamily of cytokines and is expressed in a wide range of tissues and cell types including human pancreas, small intestine, chondrocytes and neurons. There is limited information about the biological functions of IL17B, but it is known to act as a proinflammatory cytokine inducing TNFalpha and IL-1beta production in THP-1 cells and stimulating neutrophil infiltration (Bie et al. 2017, Liu et al. 2020). IL-17B has also been reported to induce the migration of CXCR4+ or CXCR5+ germinal center B cells to CXCL12 and CXCL13 (Bie et al. 2017).
IL17B is a member of the IL-17 superfamily of cytokines and is expressed in a wide range of tissues and cell types including human pancreas, small intestine, chondrocytes and neurons. There is limited information about the biological functions of IL17B, but it is known to act as a proinflammatory cytokine inducing TNFalpha and IL-1beta production in THP-1 cells and stimulating neutrophil infiltration (Bie et al. 2017, Liu et al. 2020). IL-17B has also been reported to induce the migration of CXCR4+ or CXCR5+ germinal center B cells to CXCL12 and CXCL13 (Bie et al. 2017).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,437 Da
NCBI Official Full Name
interleukin-17B
NCBI Official Synonym Full Names
interleukin 17B
NCBI Official Symbol
IL17B
NCBI Official Synonym Symbols
NIRF; IL-20; IL-17B; ZCYTO7
NCBI Protein Information
interleukin-17B; cytokine Zcyto7; cytokine-like protein ZCYTO7; interleukin 20; interleukin-17 beta; interleukin-20; neuronal interleukin-17 related factor; neuronal interleukin-17-related factor
UniProt Protein Name
Interleukin-17B
Protein Family
UniProt Gene Name
IL17B
UniProt Synonym Gene Names
IL20; NIRF; ZCYTO7; IL-17B; IL-20
UniProt Entry Name
IL17B_HUMAN
NCBI Description
The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies. [provided by RefSeq, Jul 2008]
Uniprot Description
IL17B: Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. Belongs to the IL-17 family.
Protein type: Secreted; Secreted, signal peptide; Cytokine
Chromosomal Location of Human Ortholog: 5q33.1
Cellular Component: extracellular space
Molecular Function: cytokine activity
Biological Process: cell-cell signaling; immune response; inflammatory response
Research Articles on IL-17B
Similar Products
Product Notes
The IL-17B il17b (Catalog #AAA226170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Human INTERLEUKIN-17B:Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's INTERLEUKIN-17B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 0.5ug/ml to 2.0ug/ml WB: 0.1ug/ml to 0.2ug/ml. Researchers should empirically determine the suitability of the IL-17B il17b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INTERLEUKIN-17B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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