Sheep anti-Human Integrin, beta5 Polyclonal Antibody | anti-Itgb5 antibody
Integrin, beta5
Gene Names
Itgb5; [b]5; [b]-5; [b]5A; [b]5B; beta5; ESTM23; beta-5; AA475909; AI874634
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Integrin; beta5; Polyclonal Antibody; Anti -Integrin; anti-Itgb5 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Integrin beta5. Shows <5% crossreactivity with recombinant human Integrin beta1, beta3, beta4, beta6 and recombinant mouse Integrin beta7. Species Sequence Homology: mouse; 91%, canine; 89%
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Applicable Applications for anti-Itgb5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry, Immuncytochemistry and ELISA.
Dilution: Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 50ug/ml, 10ul labels 1-2.5x10e5 MG-63 human osteosarcoma cells in a total reaction volume <200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome.
Immunocytochemistry: 10ug/ml detects Integrin, beta5 in MG-63 human osteosarcoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted I7661-43E overnight at 4 degree C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.2ng/well.
Dilution: Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 50ug/ml, 10ul labels 1-2.5x10e5 MG-63 human osteosarcoma cells in a total reaction volume <200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome.
Immunocytochemistry: 10ug/ml detects Integrin, beta5 in MG-63 human osteosarcoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted I7661-43E overnight at 4 degree C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.2ng/well.
Immunogen
CHO cell-derived recombinant human Integrin beta5, extracellular domain.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Itgb5 antibody
Integrin beta5 is a 100-110kD type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin aV. Integrin aVb5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin b5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region.
Product Categories/Family for anti-Itgb5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,052 Da
NCBI Official Full Name
integrin beta-5 isoform 1
NCBI Official Synonym Full Names
integrin beta 5
NCBI Official Symbol
Itgb5
NCBI Official Synonym Symbols
[b]5; [b]-5; [b]5A; [b]5B; beta5; ESTM23; beta-5; AA475909; AI874634
NCBI Protein Information
integrin beta-5
UniProt Protein Name
Integrin beta
Protein Family
UniProt Gene Name
Itgb5
UniProt Entry Name
Q6PE70_MOUSE
Uniprot Description
ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion
Cellular Component: cell surface; focal adhesion; membrane; leading edge; integral to membrane; integrin complex; receptor complex
Molecular Function: integrin binding; protein binding; receptor activity; receptor binding
Biological Process: integrin-mediated signaling pathway; cell-matrix adhesion; multicellular organismal development; transforming growth factor beta receptor signaling pathway; stress fiber formation; cell adhesion
Research Articles on Itgb5
Similar Products
Product Notes
The Itgb5 itgb5 (Catalog #AAA618907) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Integrin, beta5 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin, beta5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, Immuncytochemistry and ELISA. Dilution: Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions. Flow Cytometry: 50ug/ml, 10ul labels 1-2.5x10e5 MG-63 human osteosarcoma cells in a total reaction volume <200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Immunocytochemistry: 10ug/ml detects Integrin, beta5 in MG-63 human osteosarcoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted I7661-43E overnight at 4 degree C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA. Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.2ng/well. Researchers should empirically determine the suitability of the Itgb5 itgb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin, beta5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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