Western Blot (WB)
((0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Integrin beta 5 Polyclonal Antibody | anti-ITGB5 antibody
Goat anti-Integrin beta 5 Antibody
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Integrin beta 5; Polyclonal Antibody; Goat anti-Integrin beta 5 Antibody; ITGB5; integrin; beta 5; FLJ26658; anti-ITGB5 antibody
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Applicable Applications for anti-ITGB5 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 110kDa band observed in Human Ovary and Mouse Eye lysates (calculated MW of 88.1kDa according to NP_002204.2). Recommended concentration: 0.1-0.3ug/ml. Customer feedback: observed 110kDa in Mouse Eye was confirmed and not present in knock-out mouse.
Western blot: Approx 110kDa band observed in Human Ovary and Mouse Eye lysates (calculated MW of 88.1kDa according to NP_002204.2). Recommended concentration: 0.1-0.3ug/ml. Customer feedback: observed 110kDa in Mouse Eye was confirmed and not present in knock-out mouse.
Immunogen
Peptide with sequence C-TFNKFNKSYNGTVD, from the C Terminus of the protein sequence according to NP_002204.2.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
integrin beta-5
NCBI Official Synonym Full Names
integrin subunit beta 5
NCBI Official Symbol
ITGB5
NCBI Protein Information
integrin beta-5
UniProt Protein Name
Integrin beta-5
Protein Family
UniProt Gene Name
ITGB5
UniProt Entry Name
ITB5_HUMAN
Uniprot Description
ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion
Chromosomal Location of Human Ortholog: 3q21.2
Cellular Component: cell surface; focal adhesion; phagocytic vesicle; plasma membrane; receptor complex
Molecular Function: protein binding; viral receptor activity
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell-matrix adhesion; entry of virus into host cell; extracellular matrix organization and biogenesis; integrin-mediated signaling pathway; muscle contraction; stress fiber formation; transforming growth factor beta receptor signaling pathway
Research Articles on ITGB5
Similar Products
Product Notes
The ITGB5 itgb5 (Catalog #AAA421165) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Integrin beta 5 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin beta 5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 110kDa band observed in Human Ovary and Mouse Eye lysates (calculated MW of 88.1kDa according to NP_002204.2). Recommended concentration: 0.1-0.3ug/ml. Customer feedback: observed 110kDa in Mouse Eye was confirmed and not present in knock-out mouse. Researchers should empirically determine the suitability of the ITGB5 itgb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin beta 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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