Western Blot (WB)
(Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse IPF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Insulin Promoter Factor 1 Polyclonal Antibody | anti-IPF antibody
Insulin Promoter Factor 1 (IPF) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse IPF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse IPF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))
NCBI and Uniprot Product Information
Uniprot Description
PDX1: Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP. Interacts with the methyltransferase SETD7. Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 5 G3|5 86.84 cM
Cellular Component: cytoplasm; intracellular; nuclear speck; nucleoplasm; nucleus
Molecular Function: chromatin binding; DNA binding; DNA binding transcription factor activity; protein binding; protein complex binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding; transcription factor binding
Biological Process: cell differentiation; detection of glucose; endocrine pancreas development; exocrine pancreas development; glucose homeostasis; glucose metabolic process; gut development; insulin secretion; liver development; morphogenesis of embryonic epithelium; multicellular organism development; negative regulation of cell proliferation; negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of type B pancreatic cell apoptotic process; nitric oxide mediated signal transduction; pancreas development; positive regulation of cell death; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of cell proliferation; regulation of gene expression; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to glucose stimulus; smoothened signaling pathway; stem cell differentiation; transcription from RNA polymerase II promoter; transcription, DNA-dependent; type B pancreatic cell differentiation
Research Articles on IPF
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Product Notes
The IPF pdx1 (Catalog #AAA2032572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Insulin Promoter Factor 1 (IPF) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Insulin Promoter Factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the IPF pdx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Promoter Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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