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Western Blot (WB) (Western blot analysis of IGFBP2 Antibody (C-term) in Jurkat cell line lysates (35ug/lane).IGFBP2 (arrow) was detected using the purified Pab.(2ug/ml))

Rabbit anti-Human Insulin-Like Growth Factor Binding Protein 2 Polyclonal Antibody | anti-IGFBP2 antibody

Insulin-Like Growth Factor Binding Protein 2, CT (IGF-binding Protein 2, IGFBP2, IGFBP-2, BP2, IBP2, IBP-2, IGF-BP53) (AP)

Gene Names
IGFBP2; IBP2; IGF-BP53
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Insulin-Like Growth Factor Binding Protein 2; Polyclonal Antibody; CT (IGF-binding Protein 2; IGFBP2; IGFBP-2; BP2; IBP2; IBP-2; IGF-BP53) (AP); Insulin-like Growth Factor-binding Protein 2; 36kDa; anti-IGFBP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human IGFBP2.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-IGFBP2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human IGFBP2 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of IGFBP2 Antibody (C-term) in Jurkat cell line lysates (35ug/lane).IGFBP2 (arrow) was detected using the purified Pab.(2ug/ml))

Western Blot (WB) (Western blot analysis of IGFBP2 Antibody (C-term) in Jurkat cell line lysates (35ug/lane).IGFBP2 (arrow) was detected using the purified Pab.(2ug/ml))

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with IGFBP2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue with IGFBP2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of IGFBP2 Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of IGFBP2 Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of jurkat cells using IGFBP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of jurkat cells using IGFBP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-IGFBP2 antibody
IGFBP2-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Product Categories/Family for anti-IGFBP2 antibody
References
1. Arafat, A.M., et.al., J. Clin. Endocrinol. Metab. 94(12), 5093-5101 (2009). 2. Yazawa, T., et.al., Am. J. Pathol. 175(3), 976-987 (2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,814 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 2
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 2, 36kDa
NCBI Official Symbol
IGFBP2
NCBI Official Synonym Symbols
IBP2; IGF-BP53
NCBI Protein Information
insulin-like growth factor-binding protein 2; IBP-2; IGFBP-2; IGF-binding protein 2
UniProt Protein Name
Insulin-like growth factor-binding protein 2
UniProt Gene Name
IGFBP2
UniProt Synonym Gene Names
BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP-2
UniProt Entry Name
IBP2_HUMAN

Uniprot Description

IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: extracellular space; apical plasma membrane; extracellular region; cytoplasmic vesicle

Molecular Function: protein binding; insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: response to drug; response to retinoic acid; regulation of insulin-like growth factor receptor signaling pathway; response to lithium ion; response to glucocorticoid stimulus; female pregnancy; signal transduction; positive regulation of activated T cell proliferation; response to estradiol stimulus; cellular response to hormone stimulus; cellular protein metabolic process; response to mechanical stimulus; regulation of cell growth; response to nutrient; aging

Research Articles on IGFBP2

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Product Notes

The IGFBP2 igfbp2 (Catalog #AAA6481841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Insulin-Like Growth Factor Binding Protein 2, CT (IGF-binding Protein 2, IGFBP2, IGFBP-2, BP2, IBP2, IBP-2, IGF-BP53) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Insulin-Like Growth Factor Binding Protein 2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IGFBP2 igfbp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin-Like Growth Factor Binding Protein 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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