Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using INPP5B Antibody. The lane on the left was treated with blocking peptide.)
Rabbit INPP5B Polyclonal Antibody | anti-INPP5B antibody
INPP5B Antibody
Gene Names
INPP5B; 5PTase
Reactivity
Human, Mouse, RatPredicted: Bovine, Sheep
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
INPP5B; Polyclonal Antibody; INPP5B Antibody; 4; 5-trisphosphate 5-phosphatase; 5PTase; 75 kDa inositol polyphosphate 5 phosphatase; 75 kDa inositol polyphosphate-5-phosphatase; 75kDa; EC 3.1.3.36; I5P2_HUMAN; Inositol polyphosphate 5-phosphatase II; Inositol polyphosphate-5-phosphatase B; Inositol polyphosphate-5-phosphatase; Inpp5b protein; INPP5P; MGC65156; MGC71303; OTTHUMP00000004726; OTTHUMP00000004727; OTTHUMP00000004728; Phosphoinositide 5 phosphatase; Phosphoinositide 5-phosphatase; Type II inositol 1; 5 trisphosphate 5 phosphatase; Type II inositol-1; anti-INPP5B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Sheep
Predicted: Bovine, Sheep
Clonality
Polyclonal
Isotype
IgG
Specificity
INPP5B Antibody detects endogenous levels of INPP5B.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
913
Applicable Applications for anti-INPP5B antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human INPP5B.
Tissue Specificity
Platelets.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Endosome; Cytosol; Golgi Apparatus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using INPP5B Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using INPP5B Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-INPP5B antibody
Description: Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events.
Post Translational Modifications: Isoprenylation at Cys-990 may be required for localization at the membrane. May be proteolytically cleaved after Lys-320 as inferred from N-terminal protein sequence of the 75 kda form.
Subunit Structure: Interacts with APPL1, FAM109A and FAM109B. Interacts with several Rab GTPases, at least RAB1A, RAB2A, RAB5A, RAB6A, RAB8A, RAB9A and RAB33B; these interactions may play a dual role in targeting INPP5B to the specific membranes and stimulating the phosphatase activity. Interacts with INPP5F (PubMed:25869668).
Similarity: The ASH (ASPM-SPD2-Hydin) and RhoGAP (Rho GTPase activating) domains form a single folding module. The ASH domain has an immunoglobulin-like fold, the Rho-GAP domain lacks the catalytic arginine and is catalytically inactive. The ASH-RhoGAP module regulates the majority of the protein-protein interactions currently described. The ASH domain mediates association with membrane-targeting Rab GTPases. The Rho-GAP domain interacts with the endocytic adapter APPL1, which is then displaced by FAM109A and FAM109B as endosomes mature, all three interactions rely on F&H motifs, an approximately 12-13 amino-acid sequence centered around Phe and His residues essential for binding (By similarity). Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.
Post Translational Modifications: Isoprenylation at Cys-990 may be required for localization at the membrane. May be proteolytically cleaved after Lys-320 as inferred from N-terminal protein sequence of the 75 kda form.
Subunit Structure: Interacts with APPL1, FAM109A and FAM109B. Interacts with several Rab GTPases, at least RAB1A, RAB2A, RAB5A, RAB6A, RAB8A, RAB9A and RAB33B; these interactions may play a dual role in targeting INPP5B to the specific membranes and stimulating the phosphatase activity. Interacts with INPP5F (PubMed:25869668).
Similarity: The ASH (ASPM-SPD2-Hydin) and RhoGAP (Rho GTPase activating) domains form a single folding module. The ASH domain has an immunoglobulin-like fold, the Rho-GAP domain lacks the catalytic arginine and is catalytically inactive. The ASH-RhoGAP module regulates the majority of the protein-protein interactions currently described. The ASH domain mediates association with membrane-targeting Rab GTPases. The Rho-GAP domain interacts with the endocytic adapter APPL1, which is then displaced by FAM109A and FAM109B as endosomes mature, all three interactions rely on F&H motifs, an approximately 12-13 amino-acid sequence centered around Phe and His residues essential for binding (By similarity). Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 75 kDa
Predicted: 113 kDa
Predicted: 113 kDa
NCBI Official Full Name
type II inositol 1,4,5-trisphosphate 5-phosphatase isoform 1
NCBI Official Synonym Full Names
inositol polyphosphate-5-phosphatase B
NCBI Official Symbol
INPP5B
NCBI Official Synonym Symbols
5PTase
NCBI Protein Information
type II inositol 1,4,5-trisphosphate 5-phosphatase
UniProt Protein Name
Type II inositol 1,4,5-trisphosphate 5-phosphatase
Protein Family
UniProt Gene Name
INPP5B
UniProt Synonym Gene Names
OCRL2; 5PTase
UniProt Entry Name
I5P2_HUMAN
NCBI Description
This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jul 2014]
Research Articles on INPP5B
Similar Products
Product Notes
The INPP5B inpp5b (Catalog #AAA9611840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INPP5B Antibody reacts with Human, Mouse, Rat Predicted: Bovine, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's INPP5B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the INPP5B inpp5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INPP5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
