Rabbit anti-Human Inosine Monophosphate Dehydrogenase 2 Polyclonal Antibody | anti-IMPDH2 antibody

Inosine Monophosphate Dehydrogenase 2 (Inosine-5'-monophosphate Dehydrogenase 2, IMP Dehydrogenase 2, IMPDH 2, IMPDH2, IMPD 2, IMPD2, IMPDH-II) (PE)

Cat. Num. AAA6481741

• Gene Names: IMPDH2; IMPD2; IMPDH-II
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Inosine Monophosphate Dehydrogenase 2; Polyclonal Antibody; Inosine Monophosphate Dehydrogenase 2 (Inosine-5'-monophosphate Dehydrogenase 2; IMP Dehydrogenase 2; IMPDH 2; IMPDH2; IMPD 2; IMPD2; IMPDH-II) (PE); IMP (Inosine 5'-monophosphate) Dehydrogenase 2; EC=1.1.1.205; anti-IMPDH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IMPDH2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-IMPDH2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human IMPDH2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of IMPDH2 Antibody (Center) in NCI-H460,Hela,MCF7,A2058 cell line lysates(35ug/lane). IMPDH2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(IMPDH2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IMPDH2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(IMPDH2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-IMPDH2 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Sombogaard, F., Pharmacogenet. Genomics 19(8), 626-634 (2009). 2. Mohamed, M.F., Genet. Test. 12(4), 513-516 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 66933016
• NCBI GeneID: 3615
• NCBI Accession #: NP_000875
• NCBI GenBank Nucleotide #: NM_000884.2
• UniProt Accession #: P12268; Q6LEF3
• Molecular Weight: 58.0kDa (534aa)
• NCBI Official Full Name: inosine-5'-monophosphate dehydrogenase 2
• NCBI Official Synonym Full Names: inosine monophosphate dehydrogenase 2
• NCBI Official Symbol: IMPDH2
• NCBI Official Synonym Symbols: IMPD2; IMPDH-II
• NCBI Protein Information: inosine-5'-monophosphate dehydrogenase 2
• UniProt Protein Name: Inosine-5'-monophosphate dehydrogenase 2
• UniProt Gene Name: IMPDH2
• UniProt Entry Name: IMDH2_HUMAN

NCBI Description

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq, Jul 2008]

Uniprot Description

IMPDH2: a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.

Protein type: Oxidoreductase; EC 1.1.1.205; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: peroxisomal membrane; membrane; cytoplasm; nucleus; cytosol

Molecular Function: DNA binding; RNA binding; metal ion binding; nucleotide binding; IMP dehydrogenase activity

Biological Process: purine ribonucleoside monophosphate biosynthetic process; lymphocyte proliferation; nucleobase, nucleoside and nucleotide metabolic process; retina development in camera-type eye; GMP biosynthetic process; purine base metabolic process; protein homotetramerization