Rabbit anti-Human, Mouse ING4 Polyclonal Antibody | anti-ING4 antibody

ING4 Polyclonal Antibody

Cat. Num. AAA3011362

• Gene Names: ING4; my036; p29ING4
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ING4; Polyclonal Antibody; ING4 Polyclonal Antibody; Brain my036 protein; Candidate tumor suppressor p33 ING 1 homolog; Candidate tumor suppressor p33 ING1 homolog; D6Wsu147e; D6Xrf92; ING 1 like protein; ING 4; ING1 like protein; ING4_HUMAN; Inhibitor of growth family member 4; Inhibitor of growth family member 4 long isoform; Inhibitor of growth protein 4; MGC12557; my036; p29 ING 4; p29 ING4; p29ING4; anti-ING4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ING4 polyclonal antibody detects endogenous levels of ING4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 248

• Applicable Applications for anti-ING4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human ING4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ING4 polyclonal antibody)

Related Product Information for anti-ING4 antibody

Inhibitor of growth protein (ING) family of nuclear proteins, also designated ING tumor suppressor proteins, inhibit tumor progression by modulating the transcriptional outputs of signaling pathways, which in turn regulates cell proliferation. Members of this family include ING1, ING2, ING3, ING4 and INGX. ING4 localizes to the nucleus and may inhibit tumor progression by adjusting the transcriptional output of signaling pathways which regulate cell proliferation. When complexed with RELA, ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes. ING4 interacts with HIF prolyl hydroxylase 2 (EGLN1), which represses the activity of hypoxia inducible factor (HIF).

NCBI and Uniprot Product Information

• NCBI GI #: 38201670
• NCBI GeneID: 51147
• NCBI Accession #: NP_057246.2
• NCBI GenBank Nucleotide #: NP_057246.2
• UniProt Accession #: Q9UNL4
• Molecular Weight: ~ 29kDa
• NCBI Official Full Name: inhibitor of growth protein 4 isoform 1
• NCBI Official Synonym Full Names: inhibitor of growth family member 4
• NCBI Official Symbol: ING4
• NCBI Official Synonym Symbols: my036; p29ING4
• NCBI Protein Information: inhibitor of growth protein 4
• UniProt Protein Name: Inhibitor of growth protein 4
• Protein Family: Inhibitor of growth protein
• UniProt Gene Name: ING4
• UniProt Entry Name: ING4_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. Multiple alternatively spliced transcript variants have been observed that encode distinct proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

ING4: similar to ING1, a tumor suppressor protein that can interact with p53, inhibit cell growth, and induce apoptosis. Contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind p53 and p300, a component of the histone acetyl transferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. Alternatively spliced transcript variants have been observed, but the biological validity of them has not been determined.

Protein type: Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: nucleoplasm; nucleus; histone acetyltransferase complex

Molecular Function: protein binding; zinc ion binding; transcription coactivator activity; methylated histone residue binding

Biological Process: negative regulation of cell proliferation; establishment and/or maintenance of chromatin architecture; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; protein amino acid acetylation; apoptosis; positive regulation of apoptosis; DNA replication; negative regulation of transcription, DNA-dependent; cell cycle arrest; negative regulation of growth