Western Blot (WB)
(Western blot analysis of INAR2 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Rat INAR2 Polyclonal Antibody | anti-INAR2 antibody
INAR2 Antibody
Gene Names
IFNAR2; IFN-R; IMD45; IFNABR; IFNARB; IFN-alpha-REC
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
INAR2; Polyclonal Antibody; INAR2 Antibody; Human interferon alpha/beta receptor; IFN alpha REC; IFN R; IFN-a/betaR2; IFN-a/betaRbeta; IFN-alpha binding protein; IFN-alpha/beta receptor 2; IFN-R-2; IFNABR; IFNAR2; IFNARB; IFNR; INAR2_HUMAN; Interferon (alpha beta and omega) receptor 2; Interferon alpha binding protein; Interferon alpha/beta receptor 2; Interferon alpha/beta receptor beta chain; Type I interferon receptor 2; Type I interferon receptor; anti-INAR2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
INAR2 antibody detects endogenous levels of total INAR2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
331
Applicable Applications for anti-INAR2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Secreted and Membrane.
Tissue Specificity
Isoform 3 is detected in the urine (at protein level) (PubMed:8181059, PubMed:7759950). Expressed in blood cells. Expressed in lymphoblastoid and fibrosarcoma cell lines.
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of INAR2 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of INAR2 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9604049 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604049 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-INAR2 antibody
Function: Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation (PubMed:26424569). Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1 (PubMed:8181059, PubMed:7665574, PubMed:7759950). Isoform 3 is a potent inhibitor of type I IFN receptor activity (PubMed:7759950).
Subunit Structure: Heterodimer with IFNAR1 (PubMed:8181059, PubMed:7665574, PubMed:10049744, PubMed:24075985, PubMed:21854986). Isoform 1 interacts with the transcriptional factors STAT1 and STAT2 (PubMed:9121453). Interacts with JAK1 (PubMed:8181059, PubMed:7759950).
Post-translational Modifications: Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types. Glycosylated.
Similarity: Belongs to the type II cytokine receptor family.
Subunit Structure: Heterodimer with IFNAR1 (PubMed:8181059, PubMed:7665574, PubMed:10049744, PubMed:24075985, PubMed:21854986). Isoform 1 interacts with the transcriptional factors STAT1 and STAT2 (PubMed:9121453). Interacts with JAK1 (PubMed:8181059, PubMed:7759950).
Post-translational Modifications: Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types. Glycosylated.
Similarity: Belongs to the type II cytokine receptor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 58 kDa
NCBI Official Full Name
interferon alpha/beta receptor 2 isoform b
NCBI Official Synonym Full Names
interferon alpha and beta receptor subunit 2
NCBI Official Symbol
IFNAR2
NCBI Official Synonym Symbols
IFN-R; IMD45; IFNABR; IFNARB; IFN-alpha-REC
NCBI Protein Information
interferon alpha/beta receptor 2
UniProt Protein Name
Interferon alpha/beta receptor 2
UniProt Gene Name
IFNAR2
UniProt Synonym Gene Names
IFNABR; IFNARB; IFN-R-2; IFN-alpha binding protein; IFN-alpha/beta receptor 2
NCBI Description
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation (PubMed:26424569). Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1 (PubMed:8181059, PubMed:7665574, PubMed:7759950). Isoform 3 is a potent inhibitor of type I IFN receptor activity (PubMed:7759950).
Research Articles on INAR2
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Product Notes
The INAR2 ifnar2 (Catalog #AAA9604049) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INAR2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's INAR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the INAR2 ifnar2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INAR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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