Rabbit anti-Human ILK Polyclonal Antibody | anti-ILK antibody

ILK (S246) (Integrin-linked Protein Kinase, ILK-1, ILK-2, 59kD Serine/Threonine-protein Kinase, p59ILK, ILK1, ILK2) (FITC)

Cat. Num. AAA6481530

• Gene Names: ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ILK; Polyclonal Antibody; ILK (S246) (Integrin-linked Protein Kinase; ILK-1; ILK-2; 59kD Serine/Threonine-protein Kinase; p59ILK; ILK1; ILK2) (FITC); anti-ILK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ILK1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ILK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S246 of human ILK1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane). ILK(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ILK (arrow) using rabbit polyclonal ILK Antibody (pS246). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.)

Product Categories/Family for anti-ILK antibody

Antibodies; Protein Kinases

References

1. Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003). 2. Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003). 3. Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003). 4. Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003). 5. Fukuda, T., et al., J. Cell Biol. 160(7):1001-1008 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4758606
• NCBI GeneID: 3611
• NCBI Accession #: NP_004508
• NCBI GenBank Nucleotide #: NM_004517
• UniProt Accession #: Q13418
• Molecular Weight: 49kDa
• NCBI Official Full Name: integrin-linked protein kinase isoform 1
• NCBI Official Synonym Full Names: integrin linked kinase
• NCBI Official Symbol: ILK
• NCBI Official Synonym Symbols: P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• NCBI Protein Information: integrin-linked protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• Protein Family: Integrin-linked protein kinase
• UniProt Gene Name: ILK
• UniProt Synonym Gene Names: ILK1; ILK2
• UniProt Entry Name: ILK_HUMAN

NCBI Description

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; ILK subfamily

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: protein complex; costamere; focal adhesion; terminal button; intercellular junction; cytosol; nucleoplasm; sarcomere; membrane; cell soma; lamellipodium; cytoplasm; plasma membrane; stress fiber; dendritic shaft; cell junction

Molecular Function: integrin binding; protein serine/threonine kinase activity; protein binding; signal transducer activity; SH3 domain binding; protein kinase binding; ATP binding

Biological Process: negative regulation of smooth muscle cell proliferation; protein heterooligomerization; cell-matrix adhesion; positive regulation of axon extension; positive regulation of transcription, DNA-dependent; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; myelin formation; positive regulation of MAP kinase activity; positive regulation of cell proliferation; fibril organization and biogenesis; protein kinase B signaling cascade; negative regulation of neuron apoptosis; cell cycle arrest; positive regulation of cell-matrix adhesion; positive regulation of BMP signaling pathway; integrin-mediated signaling pathway; cell aging; positive regulation of dendrite morphogenesis; positive regulation of myoblast differentiation; myelination in the peripheral nervous system; establishment and/or maintenance of epithelial cell polarity; cell proliferation; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; ureteric bud branching; regulation of actin cytoskeleton organization and biogenesis; negative regulation of protein kinase activity; nerve development; positive regulation of phosphorylation; neurite morphogenesis; positive regulation of cell migration