Goat anti-Human ILF2 Polyclonal Antibody | anti-ILF2 antibody

ILF2, ID (Interleukin Enhancer-binding Factor 2, Nuclear Factor of Activated T-cells 45kD, NF45, PRO3063)

Cat. Num. AAA6010645

• Gene Names: ILF2; NF45; PRO3063
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ILF2; Polyclonal Antibody; ID (Interleukin Enhancer-binding Factor 2; Nuclear Factor of Activated T-cells 45kD; NF45; PRO3063); Anti -ILF2; anti-ILF2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human ILF2. Species sequence homology: Canine, mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-ILF2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:4000; Western Blot: 0.03-0.1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to C-EKAYEKPPEKKEG from an internal region of human ILF2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))

Testing Data

(Antibody (0.3ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-ILF2 antibody

Antibodies; Abs to Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 392513662
• NCBI GeneID: 3608
• NCBI Accession #: NP_001254738.1
• NCBI GenBank Nucleotide #: NM_001267809.1
• UniProt Accession #: Q12905; Q5SR10; Q5SR11; Q7L7R3; Q9BWD4; Q9P1N0; A6NDB0; B2R8G7
• Molecular Weight: 43,062 Da
• NCBI Official Full Name: interleukin enhancer-binding factor 2 isoform 2
• NCBI Official Synonym Full Names: interleukin enhancer binding factor 2
• NCBI Official Symbol: ILF2
• NCBI Official Synonym Symbols: NF45; PRO3063
• NCBI Protein Information: interleukin enhancer-binding factor 2; interleukin enhancer binding factor 2, 45kD; nuclear factor of activated T-cells, 45-kDa; interleukin enhancer binding factor 2, 45kDa
• UniProt Protein Name: Interleukin enhancer-binding factor 2
• Protein Family: Interleukin enhancer-binding factor
• UniProt Gene Name: ILF2
• UniProt Synonym Gene Names: NF45
• UniProt Entry Name: ILF2_HUMAN

NCBI Description

The protein encoded by this gene is the 45 kDa component of nuclear factor of activated T-cells (NFAT), a heterodimer of 45 kDa and 90 kDa proteins. NFAT is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The complex has been shown to repair DNA breaks by nonhomologous end joining and can also negatively regulate the microRNA processing pathway. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Jun 2012]

Uniprot Description

ILF2: Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.

Protein type: RNA-binding; Nucleolus; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex

Molecular Function: transferase activity; protein binding; DNA binding; double-stranded RNA binding; ATP binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; immune response