Rabbit anti-Human IL6 Polyclonal Antibody | anti-IL6 antibody

IL6, ID (IL6, IFNB2, Interleukin-6, B Cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2) (Azide free) (HRP)

Cat. Num. AAA6310293

• Gene Names: IL6; HGF; HSF; BSF2; IL-6; IFNB2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: IL6; Polyclonal Antibody; ID (IL6; IFNB2; Interleukin-6; B Cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2) (Azide free) (HRP); B-cell stimulatory factor 2; Interferon beta-2; anti-IL6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IL6.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IL6 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 27-55 in the central region of human IL6, UniProt Accession #P05231.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) using 037016. This demonstrates that 037016 detected the IL6 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in F9 whole cell lysate (20ug) using 037016 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size: 57kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: 037016 (1:2000) Lane 1: Hela whole cell lysate Lane 2: F9 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit Predicted band size: 57kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using 037016 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 037016 for immunohistochemistry.)

Product Categories/Family for anti-IL6 antibody

Antibodies; Interferon

References

1. Perez, A.B., et al. Hum. Immunol. 71(11):1135-1140(2010). 2. Weng, X.M., et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 24(1):42-44(2010). 3. Santhosh, S., et al. Trop Gastroenterol 31(1):30-33(2010). 4. Gein, O.N., et al. Patol Fiziol Eksp Ter 1, 10-13 (2010). 5. Tuttolomondo, A., et al. Cardiovasc Diabetol 9, 50 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 10834984
• NCBI GeneID: 3569
• NCBI Accession #: NP_000591.1
• NCBI GenBank Nucleotide #: NM_000600.3
• UniProt Accession #: P05231; Q9UCU2; Q9UCU3; Q9UCU4
• Molecular Weight: 212
• NCBI Official Full Name: interleukin-6
• NCBI Official Synonym Full Names: interleukin 6 (interferon, beta 2)
• NCBI Official Symbol: IL6
• NCBI Official Synonym Symbols: HGF; HSF; BSF2; IL-6; IFNB2
• NCBI Protein Information: interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor
• UniProt Protein Name: Interleukin-6
• Protein Family: Interleukin
• UniProt Gene Name: IL6
• UniProt Synonym Gene Names: IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
• UniProt Entry Name: IL6_HUMAN