Rabbit anti-Human, Mouse IL4 Polyclonal Antibody | anti-IL4 antibody

IL4, CT (IL4, Interleukin-4, B Cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra) (Biotin)

Cat. Num. AAA6310258

• Gene Names: IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: IL4; Polyclonal Antibody; CT (IL4; Interleukin-4; B Cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra) (Biotin); B-cell stimulatory factor 1; Pitrakinra; anti-IL4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IL4. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-IL4 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 122-150 in the C-terminal region of human IL4, UniProt Accession #P05112. Species sequence homology: monkey and sheep
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

((LEFT) Western Blot analysis of in WiDr cell line lysates (35ug/lane) using 037013. IL4 (arrow) was detected using the 037013. (RIGHT) Western Blot analysis in mouse cerebellum cell line lysates (35ug/lane) using 037013. IL4 (arrow) was detected using 037013.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded tonsil using 037013 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 037013 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of WiDr cells using 037013 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-IL4 antibody

Antibodies; Interleukin

References

1. Wong, H.L., et al. AIDS 24(7):1025-1033(2010). 2. Davila, S., et al. Genes Immun. 11(3):232-238(2010). 3. Trajkov, D., et al. Indian J Tuberc 56(3):117-131(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4504669
• NCBI GeneID: 3565
• NCBI Accession #: NP_000580.1
• NCBI GenBank Nucleotide #: NM_000589.3
• UniProt Accession #: P05112; Q14630
• Molecular Weight: 17,492 Da
• NCBI Official Full Name: interleukin-4 isoform 1
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• NCBI Protein Information: interleukin-4; binetrakin; pitrakinra; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; BSF-1
• UniProt Entry Name: IL4_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation; Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: regulation of isotype switching; negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; regulation of proton transport; microglial cell activation; female pregnancy; positive regulation of activated T cell proliferation; chemotaxis; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; connective tissue growth factor biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; negative regulation of chronic inflammatory response; regulation of immune response; T-helper 2 type immune response; B cell activation; regulation of phosphorylation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; positive regulation of chemokine biosynthetic process; response to ethanol; innate immune response in mucosa; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell differentiation; retina development in camera-type eye; response to cytokine stimulus; B cell differentiation; cellular defense response; positive regulation of transcription factor activity; immune response; positive regulation of transcription from RNA polymerase II promoter; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; negative regulation of apoptosis

Disease: Stroke, Ischemic