Rabbit anti-Human IL3R Polyclonal Antibody | anti-IL3R antibody

IL3R, Phosphorylated (Tyr766) (Cytokine Receptor Common beta, GM-CSF/IL-3/IL-5 Receptor Common beta Subunit, CD131, CDw131 antigen, CSF2RB, IL3RB, IL5RB) (FITC)

Cat. Num. AAA6482581

• Gene Names: CSF2RB; CD131; IL3RB; IL5RB; SMDP5; CDw131; betaGMR
• Reactivity: Human
• Applications: Western Blot, Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: IL3R; Polyclonal Antibody; Phosphorylated (Tyr766) (Cytokine Receptor Common beta; GM-CSF/IL-3/IL-5 Receptor Common beta Subunit; CD131; CDw131 antigen; CSF2RB; IL3RB; IL5RB) (FITC); anti-IL3R antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IL3R when phosphorylated at Tyr766.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-IL3R antibody: FLISA, Western Blot (WB), Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr766 of human IL3R (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-hIL3R-pY766 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-IL3R antibody

Antibodies; Interleukin

References

1. Murphy, J.M., et al., J. Biol. Chem. 279(25):26500-26508 (2004). 2. McClure, B.J., et al., Blood 101(4):1308-1315 (2003). 3. Scibek, J.J., et al., Anal. Biochem. 307(2):258-265 (2002). 4. Blake, T.J., et al., J. Leukoc. Biol. 72(6):1246-1255 (2002). 5. Evans, C.A., et al., Blood 100(9):3164-3174 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4559408
• NCBI GeneID: 1439
• NCBI Accession #: NP_000386
• NCBI GenBank Nucleotide #: NM_000395.2
• UniProt Accession #: P32927; Q5JZI1; Q6ICE0
• Molecular Weight: 49.7kDa (435aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: cytokine receptor common subunit beta
• NCBI Official Synonym Full Names: colony stimulating factor 2 receptor beta common subunit
• NCBI Official Symbol: CSF2RB
• NCBI Official Synonym Symbols: CD131; IL3RB; IL5RB; SMDP5; CDw131; betaGMR
• NCBI Protein Information: cytokine receptor common subunit beta
• UniProt Protein Name: Cytokine receptor common subunit beta
• UniProt Gene Name: CSF2RB
• UniProt Synonym Gene Names: IL3RB; IL5RB
• UniProt Entry Name: IL3RB_HUMAN

NCBI Description

The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP). [provided by RefSeq, Jul 2008]

Uniprot Description

CSF2RB: a common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. A type I cytokine family of receptor. Defects are a cause of congenital pulmonary alveolar proteinosis (PAP) [MIM:265120]. PAP is an autosomal recessive fatal respiratory disease.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: granulocyte macrophage colony-stimulating factor receptor complex; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; interleukin-5 receptor activity; interleukin-3 receptor activity; receptor activity

Biological Process: response to lipopolysaccharide; respiratory gaseous exchange; signal transduction

Disease: Surfactant Metabolism Dysfunction, Pulmonary, 5