Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using IL-2Rbeta (Phospho-Tyr364) antibody.)
Rabbit anti-Mouse IL2R beta Polyclonal Antibody | anti-IL2R beta antibody
IL2R beta antibody (Tyr364)
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
IL2R beta antibody was purified by affinity chromatography
Synonyms
IL2R beta; Polyclonal Antibody; IL2R beta antibody (Tyr364); Polyclonal IL2R beta; Anti-IL2R beta; CD122; CD122 antigen; high affinity IL-2 receptor beta subunit; High affinity IL-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; IL2RB; interleukin 2 receptor beta; Interleukin-2 receptor subunit beta; p70-75; p75; anti-IL2R beta antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
IL2R beta antibody (Tyr364) detects endogenous levels of IL2R beta only when phosphorylated at tyrosine 364. The antibody will detect species specific phosphorylation as follows: Human: Tyr367, Mouse: Tyr367
Purity/Purification
IL2R beta antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-IL2R beta antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:10000
ELISA: 1:10000
Biological Significance
IL2R beta is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit).
Cross-Reactivity
Mouse
Immunogen
IL2R beta antibody was raised in Rabbit using a synthesized phosphopeptide derived from human IL2R beta around the phosphorylation site of tyrosine 364 (Q-G-YP-F-F) as the immunogen.
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using IL-2Rbeta (Phospho-Tyr364) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using IL-2Rbeta (Phospho-Tyr364) antibody.)
Related Product Information for anti-IL2R beta antibody
Rabbit Polyclonal IL2R beta antibody (Tyr364)
Product Categories/Family for anti-IL2R beta antibody
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Product Notes
The IL2R beta (Catalog #AAA5301801) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL2R beta antibody (Tyr364) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL2R beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the IL2R beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL2R beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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