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Immunohistochemistry (IHC) (MBS9604042 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Rabbit anti-Human, Mouse IL25 Polyclonal Antibody | anti-IL25 antibody

IL25 Antibody

Gene Names
IL25; IL17E
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IL25; Polyclonal Antibody; IL25 Antibody; IL 17E; IL 25; IL-17E; IL-25; IL17e; IL25_HUMAN; Interleukin 17E; Interleukin 25; Interleukin-17E; Interleukin-25; Interleukin17E; Interleukin25; OTTHUMP00000027946; OTTHUMP00000246238; UNQ3120/PRO10272; anti-IL25 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
IL25 antibody detects endogenous levels of total IL25
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
177
Applicable Applications for anti-IL25 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Secreted.
Tissue Specificity
Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
Conjugation
Unconjugated

Immunohistochemistry (IHC)

(MBS9604042 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9604042 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of IL25 expression in ZR-75-1 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of IL25 expression in ZR-75-1 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-IL25 antibody
Function: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
Similarity: Belongs to the IL-17 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 20 kDa
Predicted: 21 kDa
NCBI Official Full Name
interleukin-25 isoform 1
NCBI Official Synonym Full Names
interleukin 25
NCBI Official Symbol
IL25
NCBI Official Synonym Symbols
IL17E
NCBI Protein Information
interleukin-25
UniProt Protein Name
Interleukin-25
Protein Family
UniProt Gene Name
IL25
UniProt Synonym Gene Names
IL17E; IL-25; IL-17E

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Research Articles on IL25

Similar Products

Product Notes

The IL25 il25 (Catalog #AAA9604042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL25 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the IL25 il25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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