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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using IL16 antibody.)

Rabbit IL16 Polyclonal Antibody | anti-IL16 antibody

IL16 (Interleukin-16, Pro-interleukin-16, Lymphocyte Chemoattractant Factor, LCF, NIL16, PRIL16, prIL-16) (PE)

Gene Names
IL16; LCF; NIL16; PRIL16; prIL-16
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
IL16; Polyclonal Antibody; IL16 (Interleukin-16; Pro-interleukin-16; Lymphocyte Chemoattractant Factor; LCF; NIL16; PRIL16; prIL-16) (PE); anti-IL16 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes IL16. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-IL16 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human IL16.
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using IL16 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using IL16 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using IL16 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of U2OS cell using IL16 antibody. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-IL16 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
141,752 Da
NCBI Official Full Name
IL16 protein
NCBI Official Synonym Full Names
interleukin 16
NCBI Official Symbol
IL16
NCBI Official Synonym Symbols
LCF; NIL16; PRIL16; prIL-16
NCBI Protein Information
pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor
UniProt Protein Name
Pro-interleukin-16
Protein Family
UniProt Gene Name
IL16
UniProt Synonym Gene Names
IL-16; LCF
UniProt Entry Name
IL16_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: viral reproduction; regulation of transcription, DNA-dependent; transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis

Research Articles on IL16

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Product Notes

The IL16 il16 (Catalog #AAA6422414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL16 (Interleukin-16, Pro-interleukin-16, Lymphocyte Chemoattractant Factor, LCF, NIL16, PRIL16, prIL-16) (PE) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IL16 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IL16 il16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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