Rabbit anti-Human IL12B Polyclonal Antibody | anti-IL12B antibody

IL12B, CT (IL12B, NKSF2, Interleukin-12 subunit beta, Cytotoxic lymphocyte maturation factor 40kD subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2) (Azide free) (HRP)

Cat. Num. AAA6309853

• Gene Names: IL12B; CLMF; NKSF; CLMF2; NKSF2; IL-12B
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IL12B; Polyclonal Antibody; CT (IL12B; NKSF2; Interleukin-12 subunit beta; Cytotoxic lymphocyte maturation factor 40kD subunit; IL-12 subunit p40; NK cell stimulatory factor chain 2) (Azide free) (HRP); NK cell stimulatory factor chain 2; anti-IL12B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IL12B antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-298 amino acids from the C-terminal region of human IL12B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(IL12B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(IL12B Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(IL12B Antibody (C-term) western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the IL12B antibody detected the IL12B protein (arrow).)

Related Product Information for anti-IL12B antibody

IL12B is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

Product Categories/Family for anti-IL12B antibody

Antibodies; Interleukin

References

Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010): Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Miteva, L.D., et al. Rheumatol. Int. (2010). In press: de Heens, G.L., et al. J. Clin. Periodontol. 37(2):129-136(2010). Sobti, R.C., et al. Folia Biol. (Praha) 56(3):110-115(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 24497438
• NCBI GeneID: 3593
• NCBI Accession #: NP_002178.2
• NCBI GenBank Nucleotide #: NM_002187.2
• UniProt Accession #: P29460
• Molecular Weight: 328
• NCBI Official Full Name: interleukin-12 subunit beta
• NCBI Official Synonym Full Names: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• NCBI Official Symbol: IL12B
• NCBI Official Synonym Symbols: CLMF; NKSF; CLMF2; NKSF2; IL-12B
• NCBI Protein Information: interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor,
• UniProt Protein Name: Interleukin-12 subunit beta
• Protein Family: Interleukin
• UniProt Gene Name: IL12B
• UniProt Synonym Gene Names: NKSF2; IL-12B; CLMF p40; NKSF2
• UniProt Entry Name: IL12B_HUMAN