Rabbit IL-5Ralpha Polyclonal Antibody | anti-IL-5Ralpha antibody

IL-5Ralpha (K68) Polyclonal Antibody

Cat. Num. AAA3003936

• Gene Names: IL5RA; IL5R; CD125; CDw125; HSIL5R3
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IL-5Ralpha; Polyclonal Antibody; IL-5Ralpha (K68) Polyclonal Antibody; Interleukin-5 receptor subunit alpha; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA; CDw125; CD125; IL5RA; IL5R; anti-IL-5Ralpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IL-5Ralpha (K68) polyclonal antibody detects endogenous levels of IL-5Ralpha protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 325

• Applicable Applications for anti-IL-5Ralpha antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human IL-5Ralpha.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of IL-5Ralpha (K68) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-IL-5Ralpha antibody

Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cellreplacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the ytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.

NCBI and Uniprot Product Information

• NCBI GI #: 191175130
• NCBI GeneID: 3568
• UniProt Accession #: Q01344
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: interleukin 5 receptor alpha transcript variant 7
• NCBI Official Synonym Full Names: interleukin 5 receptor subunit alpha
• NCBI Official Symbol: IL5RA
• NCBI Official Synonym Symbols: IL5R; CD125; CDw125; HSIL5R3
• NCBI Protein Information: interleukin-5 receptor subunit alpha
• UniProt Protein Name: Interleukin-5 receptor subunit alpha
• UniProt Gene Name: IL5RA
• UniProt Synonym Gene Names: IL5R; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA
• UniProt Entry Name: IL5RA_HUMAN

NCBI Description

The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]

Uniprot Description

IL5RA: This is the receptor for interleukin-5. The alpha chain binds to IL5. Belongs to the type I cytokine receptor family. Type 5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 3p26-p24

Cellular Component: extracellular space; plasma membrane; integral to membrane

Molecular Function: protein binding; interleukin-5 receptor activity

Biological Process: cell proliferation; regulation of interleukin-5 production; inflammatory response to antigenic stimulus; signal transduction