Rabbit IL-5 Polyclonal Antibody | anti-IL-5 antibody

Rabbit anti IL-5 Polyclonal purified

Cat. Num. AAA551046

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purification: Serum IgG fraction was purified by Protein G affinity chromatography.; Purity: >95%
• Synonyms: IL-5; Polyclonal Antibody; Rabbit anti IL-5 Polyclonal purified; TRF; il5; il-5; interleukin-5; polyclonal; pab; anti-IL-5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purification: Serum IgG fraction was purified by Protein G affinity chromatography.; Purity: >95%
• Form/Format: Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
• Sequence Length: 134

• Applicable Applications for anti-IL-5 antibody: Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)
• Application Notes: ELISA: Use at a concentration range of 1-2ug/ml.; Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.; IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
• Source Note: Polyclonal anti IL-5 was produced from sera of rabbits immunized with synthetic protein containing IL-5 C terminal antigenic determinants.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
• Presentation: Lyophilized from 0.2 um filtered solution in PBS 7.4.
• Dilution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.
• Preparation and Storage: The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.; Upon reconstitution, this antibody can be stored in working aliquots at 20 degree C for one month, or at -20 degree C for six months without detectable loss of activity. ; Avoid repeated freeze/thaw cycles.

Related Product Information for anti-IL-5 antibody

Interleukin-5 is a specific hematopoietic growth factor that is responsible for the growth and differentiation of eosinophils. IL-5 promotes the growth of immature hematopoietic progenitor cells BFU-E while it causes differentiation of CFU-E the proliferation of which is inhibited by IL-5. IL-5 strongly stimulates the proliferation, cell activation, and differentiation of eosinophils. B-cells can be made responsive to IL5 by treatment with suboptimal doses of IL1.

Product Categories/Family for anti-IL-5 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 4504671
• NCBI GeneID: 3567
• NCBI Accession #: NP_000870.1
• NCBI GenBank Nucleotide #: NM_000879.2
• UniProt Accession #: P05113; Q13840
• NCBI Official Full Name: interleukin-5
• UniProt Protein Name: Interleukin-5
• UniProt Gene Name: IL5
• UniProt Synonym Gene Names: IL-5; TRF
• UniProt Entry Name: IL5_HUMAN

NCBI Description

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation