Western Blot (WB)
(Western blot analysis of IL-36A in MCF7 cell lysate with IL-36A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Rabbit anti-Human IL-36A Polyclonal Antibody | anti-IL36A antibody
IL-36A Antibody
Gene Names
IL36A; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
IL-36A antibody is affinity chromatography purified via peptide column.
Synonyms
IL-36A; Polyclonal Antibody; IL-36A Antibody; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON); IL1E; Interleukin-36 alpha; FIL1 epsilon; IL-1 epsilon; interleukin 36; alpha; anti-IL36A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
IL-36A antibody is human specific. IL-36A antibody will not cross-react with IL-36B or IL-36G.
Purity/Purification
IL-36A antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
158
Applicable Applications for anti-IL36A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IL-36A antibody can be used for detection of IL-36A by Western blot at 1 - 2 mug/ml.
Conjugate
Unconjugated
Immunogen
IL-36A antibody was raised against an 11 amino acid peptide near the carboxy terminus of human IL-36A.
Buffer
IL-36A antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
IL-36A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of IL-36A in MCF7 cell lysate with IL-36A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of IL-36A in MCF7 cell lysate with IL-36A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of IL-36A in human brain tissue with IL-36A antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of IL-36A in human brain tissue with IL-36A antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of IL-36A in human brain tissue with IL-36A antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of IL-36A in human brain tissue with IL-36A antibody at 20 μg/mL.)
Related Product Information for anti-IL36A antibody
IL-36A is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36A is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2). Like the related proteins IL-36B and IL-36G, IL-36A requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown (3). The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 17 kDa
Observed: 16 kDa
Observed: 16 kDa
NCBI Official Full Name
interleukin-36 alpha
NCBI Official Synonym Full Names
interleukin 36, alpha
NCBI Official Symbol
IL36A
NCBI Official Synonym Symbols
FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
NCBI Protein Information
interleukin-36 alpha; FIL1 epsilon; IL-1 epsilon; interleukin-1 epsilon; IL-1F6 (FIL-1-epsilon); interleukin 1, epsilon; interleukin-1 family member 6; interleukin 1 family, member 6 (epsilon)
UniProt Protein Name
Interleukin-36 alpha
Protein Family
UniProt Gene Name
IL36A
UniProt Synonym Gene Names
FIL1E; IL1E; IL1F6; IL-1 epsilon; IL-1F6
UniProt Entry Name
IL36A_HUMAN
Uniprot Description
IL1F6: Belongs to the IL-1 family.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 2q12-q14.1
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-1 receptor binding; cytokine activity
Biological Process: cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of interleukin-6 production; immune response; inflammatory response
Research Articles on IL36A
Similar Products
Product Notes
The IL36A il36a (Catalog #AAA153528) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL-36A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-36A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). IL-36A antibody can be used for detection of IL-36A by Western blot at 1 - 2 mug/ml. Researchers should empirically determine the suitability of the IL36A il36a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-36A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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