Rabbit IKKgamma Polyclonal Antibody | anti-IKKgamma antibody

IKKgamma Polyclonal Antibody

Cat. Num. AAA3012882

• Gene Names: IKBKG; IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; EDAID1; IKKAP1; ZC2HC9; IKK-gamma
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: IKKgamma; Polyclonal Antibody; IKKgamma Polyclonal Antibody; NEMO; IKKAP1; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; IKBKG; FIP3; IkB kinase associated protein 1; Inhibitor of nuclear factor kappa B kinase subunit gamma; AMCBX1; FIP 3; FIP-3; Fip3p; I kappa B kinase gamma; IkB kinase gamma subunit; IkB kinase-associated protein 1; Ikbkg; IMD33; Incontinentia pigmenti; Inhibitor of kappa light polypeptide gene enhancer in B cells; kinase gamma; kinase of; gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IP; IP1; IP2; IPD2; NF kappa B essential modifier; NF kappa B essential modulator; NF-kappa-B essential modifier; NF-kappa-B essential modulator; ZC2HC9; anti-IKKgamma antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IKKgamma polyclonal antibody detects endogenous levels of IKKgamma protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 487

• Applicable Applications for anti-IKKgamma antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:50-1:200; IHC: 1:20-1:50; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of IKK gamma on Hela cell using anti-IKK gamma antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining IKK gamma in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-IKKgamma antibody

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.

NCBI and Uniprot Product Information

• NCBI GI #: 223671870
• NCBI GeneID: 8517
• NCBI Accession #: NP_001093326.2
• NCBI GenBank Nucleotide #: NP_001093326.2
• UniProt Accession #: Q9Y6K9; O88522; Q6TMG5
• Molecular Weight: 48kDa
• NCBI Official Full Name: NF-kappa-B essential modulator isoform b
• NCBI Official Synonym Full Names: inhibitor of nuclear factor kappa B kinase regulatory subunit gamma
• NCBI Official Symbol: IKBKG
• NCBI Official Synonym Symbols: IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; EDAID1; IKKAP1; ZC2HC9; IKK-gamma
• NCBI Protein Information: NF-kappa-B essential modulator
• UniProt Protein Name: NF-kappa-B essential modulator
• UniProt Gene Name: IKBKG
• UniProt Synonym Gene Names: FIP3; NEMO; NEMO; IKKAP1; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG
• UniProt Entry Name: NEMO_HUMAN

NCBI Description

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]

Uniprot Description

IKKG: a regulatory subunit of the IKK-signalosome complex. Interacts preferentially with IKK-beta but also able to interact with IKK-alpha, IKAP, TAX, RIP and MAP3K14/NIK. Defects are the cause of familial incontinentia pigmenti type II (IP2).

Protein type: Protein kinase, regulatory subunit; Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: spindle pole; cytoplasm; intracellular; IkappaB kinase complex; nucleus; cytosol; ubiquitin ligase complex

Molecular Function: protein domain specific binding; signal transducer activity; peroxisome proliferator activated receptor binding; protein binding; protein homodimerization activity; protein heterodimerization activity; metal ion binding; ubiquitin protein ligase binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; establishment of vesicle localization; viral reproduction; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; B cell homeostasis; positive regulation of interferon type I production; JNK cascade; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; response to virus; activation of NF-kappaB-inducing kinase; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; toll-like receptor 9 signaling pathway; response to DNA damage stimulus

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 2; Ectodermal Dysplasia, Hypohidrotic, With Immune Deficiency; Immunodeficiency Without Anhidrotic Ectodermal Dysplasia; Immunodeficiency 33; Incontinentia Pigmenti; Ectodermal Dysplasia, Anhidrotic, With Immunodeficiency, Osteopetrosis, And Lymphedema