Rabbit anti-Human IKKB Polyclonal Antibody | anti-IKKB antibody

IKKB, Phosphorylated (S705) (IKBKB, IKKB, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta) (Azide free) (HRP)

Cat. Num. AAA6309776

• Gene Names: IKBKB; IKK2; IKKB; NFKBIKB; IKK-beta
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IKKB; Polyclonal Antibody; Phosphorylated (S705) (IKBKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta) (Azide free) (HRP); IKBKB; Nuclear factor NF-kappa-B inhibitor kinase beta; anti-IKKB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IKKB antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-IKKB-S705 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S705 of human IKKB.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-IKKB-S705 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-IKKB antibody

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Product Categories/Family for anti-IKKB antibody

Antibodies; Protein Kinases

References

Yatherajam, G., et al. J. Immunol. 185(5):2665-2669(2010). Kenneth, N.S., et al. EMBO J. 29(17):2966-2978(2010). Zhao, M., et al. J. Biol. Chem. 285(32):24372-24380(2010). Niida, M., et al. Mol. Immunol. 47(14):2378-2387(2010). Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 299758411
• NCBI GeneID: 3551
• NCBI Accession #: NP_001177649.1
• NCBI GenBank Nucleotide #: NM_001190720.2
• UniProt Accession #: O14920; O75327; B4DZ30; B4E0U4
• Molecular Weight: 86,564 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta isoform 2
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• NCBI Official Symbol: IKBKB
• NCBI Official Synonym Symbols: IKK2; IKKB; NFKBIKB; IKK-beta
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta; IKK-B; I-kappa-B kinase 2; I-kappa-B-kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Gene Name: IKBKB
• UniProt Synonym Gene Names: IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
• UniProt Entry Name: IKKB_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

Protein type: EC 2.7.11.10; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: internal side of plasma membrane; cytoplasm; IkappaB kinase complex; nucleus; cytosol; lipid raft

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; response to virus; positive regulation of transcription, DNA-dependent; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; serine phosphorylation of STAT protein; B cell homeostasis; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; I-kappaB phosphorylation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

Disease: Immunodeficiency 15