Rabbit anti-Human IKK-gamma Polyclonal Antibody | anti-IKK-gamma antibody

IKK-gamma (Phospho-Ser376) Antibody

Cat. Num. AAA9503253

• Gene Names: IKBKG; IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: IKK-gamma; Polyclonal Antibody; IKK-gamma (Phospho-Ser376) Antibody; NEMO; NF-kappa-B essential modifier; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IkB kinase subunit gamma; I-kappa-B kinase gamma; IKKG; IkB kinase-associated protein 1; IKKAP1; FIP-3; anti-IKK-gamma antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: IKK-gamma (Phospho-Ser376) Antibody detects endogenous levels of IKK-gamma only when phosphorylated at Ser376.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 320

• Applicable Applications for anti-IKK-gamma antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50~1:100; ELISA: 1:1000
• Target Modification: Phospho
• Modification Sites: H:S376
• Immunogen: The antiserum was produced against synthesized peptide derived from human IKK-gamma around the phosphorylation site of Ser376.
• Immunogen Range: 342-391
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain, using IKK-gamma (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 223671870
• NCBI GeneID: 8517
• NCBI Accession #: NP_001093326.2
• NCBI GenBank Nucleotide #: NM_001099856.4
• UniProt Accession #: Q9Y6K9; Q7LBY6; Q7Z7F1
• Molecular Weight: 48 kDa
• NCBI Official Full Name: NF-kappa-B essential modulator isoform b
• NCBI Official Synonym Full Names: inhibitor of nuclear factor kappa B kinase subunit gamma
• NCBI Official Symbol: IKBKG
• NCBI Official Synonym Symbols: IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma
• NCBI Protein Information: NF-kappa-B essential modulator
• UniProt Protein Name: NF-kappa-B essential modulator
• UniProt Gene Name: IKBKG
• UniProt Synonym Gene Names: FIP3; NEMO; NEMO; IKKAP1; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma

NCBI Description

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]

Uniprot Description

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.