Rabbit anti-Human, Mouse IKK beta Polyclonal Antibody | anti-IKKb antibody

IKK beta (IkappaB Kinase beta, IKKb, IKK2) (FITC)

Cat. Num. AAA6481442

• Gene Names: IKBKB; IKK2; IKKB; NFKBIKB; IKK-beta
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: IKK beta; Polyclonal Antibody; IKK beta (IkappaB Kinase beta; IKKb; IKK2) (FITC); anti-IKKb antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IKK beta. Does not cross react with IKK alpha or IKK gama. Species Crossreactivity: mouse.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-IKKb antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1ug/ml; WB: 1:500-1:1000 (1ug/ml); Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to aa662-680 of human IKK beta, which differs from the corresponding murine sequence by one amino acid. Cellular Localization: Cytoplasm.
• Conjugate: FITC
• Positive Control: A431 cell lysate, HeLa cells
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot of A431 lysate using II3000-26A (1:500). Proteins were visualized using a IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system.)

Western Blot (WB)

(Western Blot using I3000-26A (1:500) Lane 1: C2C12 lysate Lane 2 A431 lysate Were resolved by electrophoresis, transferred to PVDF membrane and. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.)

Western Blot (WB)

(Western Blot using I3000-26A. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.)

Immunohistochemistry (IHC)

(IKKbeta staining on HeLa cells, pretreated with citrate buffer, pH 6.0. A previous lot of I3000-26A was used at 1ug/ml, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected as nuclear in tumor cells.)

Immunohistochemistry (IHC)

(Negative control on HeLa cells, pretreated with citrate buffer, pH 6.0. Antibody diluent incubation, IHC-Select detection with HRP-DAB. No signal was detected.)

Product Categories/Family for anti-IKKb antibody

Antibodies; Protein Kinases

References

1. Nakano, H., et al. (1998). Proc. Natl. Acad. Sci. USA. 95: 3537-42 2. DiDonato, J.A., et al. (1997). Nature. 388: 548-54. 3. Regnier, C.H., et al. (1997). Cell. 90: 373-383. 4. Zandi, E. et al. (1997). Cell. 91: 243-52. 5. Woronicz, J.D., et al. (1997). Science. 278: 866-9. 6. Mercuiro, F., et al. (1997). Science. 278: 860-6.

NCBI and Uniprot Product Information

• NCBI GI #: 299758411
• NCBI GeneID: 3551
• NCBI Accession #: NP_001177649.1
• NCBI GenBank Nucleotide #: NM_001556.1
• UniProt Accession #: O14920; O75327; B4DZ30; B4E0U4
• Molecular Weight: 86,564 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta isoform 2
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• NCBI Official Symbol: IKBKB
• NCBI Official Synonym Symbols: IKK2; IKKB; NFKBIKB; IKK-beta
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta; IKK-B; I-kappa-B kinase 2; I-kappa-B-kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Gene Name: IKBKB
• UniProt Synonym Gene Names: IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
• UniProt Entry Name: IKKB_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

Protein type: EC 2.7.11.10; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: internal side of plasma membrane; cytoplasm; IkappaB kinase complex; nucleus; cytosol; lipid raft

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; response to virus; positive regulation of transcription, DNA-dependent; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; serine phosphorylation of STAT protein; B cell homeostasis; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; I-kappaB phosphorylation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

Disease: Immunodeficiency 15