Rabbit anti-Human IkB alpha Polyclonal Antibody | anti-IKBA, antibody

IkB alpha Polyclonal Antibody

Cat. Num. AAA2556110

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI; EDAID2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: IkB alpha; Polyclonal Antibody; IkB alpha Polyclonal Antibody; I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; anti-IKBA; antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 100 mug/ml BSA and 50% glycerol.
• Concentration: 1.3 mg/mL (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-IKBA, antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:500-1:1000
• Immunogen: KLH conjugated synthetic peptide corresponding to Mouse IKBalpha
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung cancer using IkB alpha Polyclonal Antibody at dilution of 1:500.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human stomach cancer using IkB alpha Polyclonal Antibody at dilution of 1:500.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon cancer using IkB alpha Polyclonal Antibody at dilution of 1:500.)

Related Product Information for anti-IKBA, antibody

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Product Categories/Family for anti-IKBA, antibody

Cancer; Epigenetics and Nuclear Signaling; Immunology; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NM_020529.2
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 35,609 Da
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: NFKB inhibitor alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI; EDAID2
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.