Western Blot (WB)
(IGSF5 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the IGSF5 antibody detected the IGSF5 protein (arrow).)
Rabbit anti-Human IGSF5 Polyclonal Antibody | anti-IGSF5 antibody
IGSF5, NT (IGSF5, JAM4, Immunoglobulin superfamily member 5, Junctional adhesion molecule 4) (Azide free) (HRP)
Gene Names
IGSF5; GSF5; JAM4
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
IGSF5; Polyclonal Antibody; NT (IGSF5; JAM4; Immunoglobulin superfamily member 5; Junctional adhesion molecule 4) (Azide free) (HRP); Junctional adhesion molecule 4; anti-IGSF5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-IGSF5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
IGSF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human IGSF5.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(IGSF5 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the IGSF5 antibody detected the IGSF5 protein (arrow).)
Western Blot (WB)
(IGSF5 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the IGSF5 antibody detected the IGSF5 protein (arrow).)
Related Product Information for anti-IGSF5 antibody
Provides, together with MAGI1, an adhesion machinery at tight junctions, which may regulate the permeability of kidney glomerulus and small intestinal epithelial cells. Mediates calcium-independent homophilic cell adhesion. In testis, it may function as a cell adhesion molecule rather than a tight-junction protein. It may participate in the adhesion between spermatogonia-spermatogonia, spermatogonia-Sertoli cells, and Sertoli cells-Sertoli cells (By similarity).
Product Categories/Family for anti-IGSF5 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,593 Da
NCBI Official Full Name
immunoglobulin superfamily member 5
NCBI Official Synonym Full Names
immunoglobulin superfamily, member 5
NCBI Official Symbol
IGSF5
NCBI Official Synonym Symbols
GSF5; JAM4
NCBI Protein Information
immunoglobulin superfamily member 5; JAM-4; junctional adhesion molecule 4; immunoglobulin superfamily 5 like
UniProt Protein Name
Immunoglobulin superfamily member 5
Protein Family
UniProt Gene Name
IGSF5
UniProt Synonym Gene Names
JAM4; IgSF5; JAM-4
UniProt Entry Name
IGSF5_HUMAN
Uniprot Description
IGSF5: Provides, together with MAGI1, an adhesion machinery at tight junctions, which may regulate the permeability of kidney glomerulus and small intestinal epithelial cells. Mediates calcium-independent homophilic cell adhesion. In testis, it may function as a cell adhesion molecule rather than a tight-junction protein. It may participate in the adhesion between spermatogonia- spermatogonia, spermatogonia-Sertoli cells, and Sertoli cells- Sertoli cells. Belongs to the immunoglobulin superfamily.
Protein type: Membrane protein, integral; Cell surface; Immunoglobulin superfamily; Cell adhesion
Chromosomal Location of Human Ortholog: 21q22.2
Cellular Component: neuron projection; tight junction; cell surface; cell soma; integral to membrane; plasma membrane; intercellular junction
Molecular Function: PDZ domain binding
Biological Process: cell-cell adhesion; regulation of neurogenesis
Research Articles on IGSF5
Similar Products
Product Notes
The IGSF5 igsf5 (Catalog #AAA6309589) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IGSF5, NT (IGSF5, JAM4, Immunoglobulin superfamily member 5, Junctional adhesion molecule 4) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IGSF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IGSF5 igsf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGSF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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