Rabbit anti-Sheep IgG (H&L) Polyclonal Antibody
Rabbit anti Sheep IgG (H&L)
Reactivity
Sheep
Purity
Isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads
Synonyms
IgG (H&L); Polyclonal Antibody; Rabbit anti Sheep IgG (H&L); Rabbit Antibody to Sheep Immunoglobulin G (IgG); Heavy & Light chains; anti-IgG (H&L) antibody
Host
Rabbit
Reactivity
Sheep
Clonality
Polyclonal
Specificity
IgG (H&L)
Based on immunoelectrophoresis, the product reacts with the heavy chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but antibodies may cross-rea
Based on immunoelectrophoresis, the product reacts with the heavy chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but antibodies may cross-rea
Purity/Purification
Isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads
Form/Format
HRP, Lyophilized
Reconstitute to 1.5ml with distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette.
Reconstitute to 1.5ml with distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette.
Concentration
0.8mg/ml (prior to lyophilization) (varies by lot)
Sequence Length
128
Application Notes
A dilution range of 1:500 - 1:5,000 is suggested for immunohistochemistry on tissue section, 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates and 1:10,000 - 1:200,000 for Western blotting with ECL substrates. Each laboratory
Buffer
Lyophilized from 0.01M Sodium phosphate, 0.25M Sodium chloride, pH 7.6 containing 15 mg/ml BSA
Preservative
None. (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Lyophilized
Yes
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
Bovine serum albumin (BSA) and dry milk may contain IgG. Secondary antibodies directed against bovine, goat, horse, or sheep react with bovine IgG. Therefore, use of BSA or milk to block or dilute these antibodies may significantly increase back
Preparation and Storage
Store lyophilized product at 2 to 8 degree C. After reconstitution, product is stable for several weeks at 2 to 8 degree C as an undiluted liquid. Prepare working dilution only prior to immediate use. For extended storage after reconstitution, we suggest
Product Categories/Family for anti-IgG (H&L) antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
IgG
Similar Products
Product Notes
The IgG (H&L) (Catalog #AAA316541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Sheep IgG (H&L) reacts with Sheep and may cross-react with other species as described in the data sheet. A dilution range of 1:500 - 1:5,000 is suggested for immunohistochemistry on tissue section, 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates and 1:10,000 - 1:200,000 for Western blotting with ECL substrates. Each laboratory. Researchers should empirically determine the suitability of the IgG (H&L) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (H&L), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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