Testing Data #1
(Inhibition of Adalimumab binding to TNFα by HCA202. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (HCA202) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using Goat anti-human Fab (MBS223909) )
Goat IgG Polyclonal Antibody | anti-IgG F(ab')2 antibody
GOAT ANTI HUMAN IgG F(ab')2:HRP
Applications
ELISA, Western Blot
Synonyms
IgG; Polyclonal Antibody; GOAT ANTI HUMAN IgG F(ab')2:HRP; anti-IgG F(ab')2 antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Form/Format
HRP
Purified IgG - liquid
Purified IgG - liquid
Concentration
Approximate Protein Concentration: 0.5 mg/ml (varies by lot)
Sequence Length
106
Applicable Applications for anti-IgG F(ab')2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000;
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000
Perservative Stabilisers
0.01% Thiomersal
1% Bovine Serum Albumin
Preparation
1% Bovine Serum Albumin
Preparation
Immunogen
Human IgG F(ab')2
Buffer Solution
Buffer Solution
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Inhibition of Adalimumab binding to TNFα by HCA202. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (HCA202) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using Goat anti-human Fab (MBS223909) )
Testing Data #1
(Inhibition of Adalimumab binding to TNFα by HCA202. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (HCA202) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using Goat anti-human Fab (MBS223909) )
Related Product Information for anti-IgG F(ab')2 antibody
Goat anti Human IgG F(ab')2 antibody recognizes Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity. Goat anti Human IgG F(ab')2 antibody demonstrates minimal cross reactivity with bovine, horse and mouse IgG; other species have not been tested.No reactivity is seen with the Fc portion of human IgG or non-immunoglobulin serum proteins. This antibody is a performance tested, matched reagent for use with MyBioSource HuCAL antibodies.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
11,609 Da
NCBI Official Full Name
Ig kappa chain C region, partial
UniProt Protein Name
Ig kappa chain C region
Protein Family
UniProt Gene Name
IGKC
UniProt Entry Name
IGKC_HUMAN
Uniprot Description
IGKC: Defects in IGKC are the cause of immunoglobulin kappa light chain deficiency (IGKCD). IGKCD is a disease characterized by the complete absence of immunoglobulin kappa chains.
Chromosomal Location of Human Ortholog: 2p12
Cellular Component: extracellular space; plasma membrane; extracellular region
Molecular Function: antigen binding
Biological Process: retinal homeostasis; receptor-mediated endocytosis; regulation of immune response; innate immune response; immune response; complement activation, classical pathway; complement activation
Similar Products
Product Notes
The IgG F(ab')2 igkc (Catalog #AAA223909) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000; Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000. Researchers should empirically determine the suitability of the IgG F(ab')2 igkc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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