Rabbit IGF2R / CD222 Polyclonal Antibody | anti-IGF2R antibody

Rabbit Polyclonal (IgG) to Human IGF2R / CD222

Cat. Num. AAA243038

• Gene Names: IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: IGF2R / CD222; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human IGF2R / CD222; Anti-IGF2R / CD222 Antibody (aa2441-2490) IHC-plus; IGF2R; CD222 antigen; CIMPR; CI Man-6-P receptor; CI-MPR; IGF-II receptor; MPR 300; MPR1; MPRI; M6P-R; M6P/IGF2R; CD222; M6P/IGF2 receptor; Human IGF2R; anti-IGF2R antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IGF2R Antibody detects endogenous levels of total IGF2R protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2491

• Applicable Applications for anti-IGF2R antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: ELISA (1:20000), IF (1:100 - 1:500), IHC-P (10 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human IGF2R (aa2441-2490).
• Immunogen: IGF2R / CD222 antibody was raised against synthetic peptide from human IGF2R (aa2441-2490).
• Antigen Modification: aa2441-2490
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-IGF2R antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-IGF2R antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-IGF2R antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of HeLa cells, using IGF2R Antibody. The picture on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 119964726
• NCBI GeneID: 3482
• NCBI Accession #: NP_000867.2
• NCBI GenBank Nucleotide #: NM_000876.2
• UniProt Accession #: P11717; Q7Z7G9; Q96PT5
• Molecular Weight: 274,375 Da
• NCBI Official Full Name: cation-independent mannose-6-phosphate receptor
• NCBI Official Synonym Full Names: insulin-like growth factor 2 receptor
• NCBI Official Symbol: IGF2R
• NCBI Official Synonym Symbols: MPR1; MPRI; CD222; CIMPR; M6P-R
• NCBI Protein Information: cation-independent mannose-6-phosphate receptor; M6PR; CI-MPR; MPR 300; M6P/IGF2R; IGF-II receptor; M6P/IGF2 receptor; CI Man-6-P receptor; 300 kDa mannose 6-phosphate receptor; insulin-like growth factor II receptor; cation-independent mannose-6 phosphate receptor
• UniProt Protein Name: Cation-independent mannose-6-phosphate receptor
• Protein Family: Cation-independent mannose-6-phosphate receptor
• UniProt Gene Name: IGF2R
• UniProt Synonym Gene Names: MPRI; CI Man-6-P receptor; CI-MPR; M6PR; MPR 300; IGF-II receptor; M6P/IGF2R
• UniProt Entry Name: MPRI_HUMAN

NCBI Description

This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele. [provided by RefSeq, Apr 2013]

Uniprot Description

IGF2R: a multifunctional type I transmembrane protein receptor that binds insulin-like growth factor 2 at the cell surface and mannose-6-phosphate-tagged proteins in the trans-Golgi network. Also known as the cation-independent mannose-6 phosphate (M6P) receptor. Clears IGF2 from the cell surface to attenuate signaling, and transports M6P-tagged lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. M6P-tagged lysosomal enzymes bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Belongs to the MRL1/IGF2R family.

Protein type: Vesicle; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: extracellular space; focal adhesion; cell surface; clathrin coat; lysosomal membrane; integral to plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle; membrane; endocytic vesicle; nuclear envelope lumen; perinuclear region of cytoplasm; late endosome; endosome

Molecular Function: mannose binding; identical protein binding; retinoic acid binding; transporter activity; phosphoprotein binding; G-protein alpha-subunit binding; receptor activity; insulin-like growth factor II binding; insulin-like growth factor receptor activity; G-protein coupled receptor activity; protein binding; enzyme binding; glycoprotein binding

Biological Process: receptor-mediated endocytosis; G-protein coupled receptor protein signaling pathway; organ regeneration; response to retinoic acid; insulin-like growth factor receptor signaling pathway; positive regulation of apoptosis; spermatogenesis; signal transduction; liver development; post-embryonic development

Disease: Hepatocellular Carcinoma