Rabbit anti-Human, Mouse IGF2BP3 Polyclonal Antibody | anti-IGF2BP3 antibody

IGF2BP3 Antibody

Cat. Num. AAA9630771

• Gene Names: IGF2BP3; CT98; IMP3; KOC1; IMP-3; VICKZ3
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: IGF2BP3; Polyclonal Antibody; IGF2BP3 Antibody; Cancer/testis antigen 98; CT98; DKFZp686F1078; hKOC; IF2B3_HUMAN; IGF II mRNA binding protein 3; IGF-II mRNA-binding protein 3; IGF2 mRNA binding protein 3; IGF2 mRNA-binding protein 3; IMP 3; IMP-3; Insulin like growth factor 2 mRNA binding protein 3; Insulin-like growth factor 2 mRNA-binding protein 3; KH domain containing protein overexpressed in cancer; KH domain-containing protein overexpressed in cancer; KOC 1; KOC1; VICKZ 3; VICKZ family member 3; VICKZ3; anti-IGF2BP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: IGF2BP3 Antibody detects endogenous levels of IGF2BP3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-IGF2BP3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human IGF2BP3.
• Conjugation: Unconjugated
• Similarity: All KH domains contribute binding to target mRNA. They are also required for RNA-dependent homo- and heterooligomerization. The integrity of KH domains seems not to be required for localization to stress granules. Belongs to the RRM IMP/VICKZ family.
• Subunit Structure: Can form homooligomers and heterooligomers with IGF2BP1 and IGF2BP3 in an RNA-dependent manner. Interacts with IGF2BP1. Interacts with ELAVL1, DHX9 and HNRNPU.
• Tissue Specificity: Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
• Function: RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
• Note: Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules. In response to cellular stress, such as oxidative stress, recruited to stress granules.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-IGF2BP3 antibody

Nucleus; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 30795212
• NCBI GeneID: 10643
• NCBI Accession #: NP_006538.2
• NCBI GenBank Nucleotide #: NM_006547.2
• UniProt Accession #: O00425; Q63HM0; Q6MZZ2; Q86VB1; A0A4Z5
• Molecular Weight: 63,705 Da
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 3
• NCBI Official Synonym Full Names: insulin-like growth factor 2 mRNA binding protein 3
• NCBI Official Symbol: IGF2BP3
• NCBI Official Synonym Symbols: CT98; IMP3; KOC1; IMP-3; VICKZ3
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 3; hKOC; VICKZ family member 3; cancer/testis antigen 98; IGF2 mRNA-binding protein 3; IGF II mRNA binding protein 3; IGF-II mRNA-binding protein 3; KH domain containing protein overexpressed in cancer; KH
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 3
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP3
• UniProt Synonym Gene Names: IMP3; KOC1; VICKZ3; IGF2 mRNA-binding protein 3; IMP-3; hKOC
• UniProt Entry Name: IF2B3_HUMAN

NCBI Description

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]

Uniprot Description

IGF2BP3: RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. Belongs to the RRM IMP/VICKZ family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; Translation

Chromosomal Location of Human Ortholog: 7p11

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; mRNA 3'-UTR binding; RNA binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: anatomical structure morphogenesis; mRNA transport; translation; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process