Sheep anti-Human IgA, Secretory Component, Free and Bound Polyclonal Antibody
IgA, Secretory Component, Free and Bound (sIgA, Secretory IgA)
Reactivity
Human
Applications
ELISA
Purity
Purified
Synonyms
IgA; Secretory Component; Free and Bound; Polyclonal Antibody; Free and Bound (sIgA; Secretory IgA); Anti -IgA; anti-IgA; Free and Bound antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human IgA Secretory Component. Reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal human milk, using a high electroendosmosis agar plate, free secretory component is precipitated in the alpha-2 region. Does not react with other molecular forms of IgA, or with any other secretory or plasma protein.
Purity/Purification
Purified
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
Concentration
~10 mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-IgA, Secretory Component, Free and Bound antibody
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA, Western Blot, Dot Blot and Indirect Immunofluorescence. Other application not tested.
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Immunogen
Purified free human Secretory Component isolated from pooled milk.
Grade
Purified
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-IgA, Secretory Component, Free and Bound antibody
Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. Secretory component also has an affinity for polymeric IgM.
Product Categories/Family for anti-IgA, Secretory Component, Free and Bound antibody
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Product Notes
The IgA, Secretory Component, Free and Bound (Catalog #AAA646695) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The IgA, Secretory Component, Free and Bound (sIgA, Secretory IgA) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgA, Secretory Component, Free and Bound can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA, Western Blot, Dot Blot and Indirect Immunofluorescence. Other application not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the IgA, Secretory Component, Free and Bound for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgA, Secretory Component, Free and Bound, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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