Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 94.3 kDa IFT88 protein using Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 94.3 kDa. Other Band(s): 45 kDa.)
Rabbit anti-Human; Mouse IFT88 Polyclonal Antibody | anti-IFT88 antibody
IFT88 Antibody: ATTO 594
Gene Names
IFT88; DAF19; TG737; TTC10; hTg737; D13S1056E
Reactivity
Human; Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Peptide Affinity Purified
Synonyms
IFT88; Polyclonal Antibody; IFT88 Antibody: ATTO 594; Rabbit Anti-Human IFT88 Polyclonal; D13S1056E Antibody; TPR repeat protein 10 Antibody; Tetratricopeptide repeat domain 10 Antibody; Ift88 Antibody; Recessive polycystic kidney disease protein Tg737 homolog Antibody; IFT88_HUMAN Antibody; Polaris homolog Antibody; Probe hTg737 (polycystic kidney disease; autosomal recessive) Antibody; TG737 Antibody; TTC10 Antibody; DAF19 Antibody; IFT88 Antibody; hTg737 Antibody; Intraflagellar transport 88 homolog Antibody; MGC26259 Antibody; Tetratricopeptide repeat protein 10 Antibody; Intraflagellar transport protein 88 homolog Antibody; RP11-172H24.2 Antibody; anti-IFT88 antibody
Host
Rabbit
Reactivity
Human; Mouse
Clonality
Polyclonal
Specificity
Detects ~94 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
805
Applicable Applications for anti-IFT88 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1000
ICC/IF: 1:100
ICC/IF: 1:100
Target
IFT88
Immunogen
Synthetic peptide from the mid to C-terminal of Human IFT88 (aa. 674-686)
Immunogen Species
Human
Cellular Localization
Cytoplasm; Cytoskeleton; Microtubule Organizing Center; Centrosome; Centriole; Cytoplasm; Cytoskeleton; Cilium Basal Body
Conjugate
ATTO 594
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 94.3 kDa IFT88 protein using Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 94.3 kDa. Other Band(s): 45 kDa.)
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 94.3 kDa IFT88 protein using Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 94.3 kDa. Other Band(s): 45 kDa.)
Product Categories/Family for anti-IFT88 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,381 Da
NCBI Official Full Name
intraflagellar transport protein 88 homolog isoform 3
NCBI Official Synonym Full Names
intraflagellar transport 88
NCBI Official Symbol
IFT88
NCBI Official Synonym Symbols
DAF19; TG737; TTC10; hTg737; D13S1056E
NCBI Protein Information
intraflagellar transport protein 88 homolog
UniProt Protein Name
Intraflagellar transport protein 88 homolog
Protein Family
UniProt Gene Name
IFT88
UniProt Synonym Gene Names
TG737; TTC10; TPR repeat protein 10
NCBI Description
This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2017]
Uniprot Description
Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
Research Articles on IFT88
Similar Products
Product Notes
The IFT88 ift88 (Catalog #AAA809980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFT88 Antibody: ATTO 594 reacts with Human; Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IFT88 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000 ICC/IF: 1:100. Researchers should empirically determine the suitability of the IFT88 ift88 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFT88, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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