Immunohistochemistry (IHC)
(Immunochemical staining of human IFITM1 in human tonsil with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Human IFITM1/CD225 Polyclonal Antibody | anti-IFITM1 antibody
Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal
IHC-P: 1:10,000-1:50,000
IP: 0.2-1uL/mg of lysate
Immunohistochemistry (IHC)
(Immunochemical staining of human IFITM1 in human tonsil with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human IFITM1 in human tonsil with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human IFITM1 in human colon carcinoma with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(CD225 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate0.5 uL anti-CD225 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD225 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
Immunoprecipitation (IP)
(CD225 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate0.5 uL anti-CD225 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD225 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
Western Blot (WB)
(Anti-IFITM1 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:15 kDa)
Western Blot (WB)
(Anti-IFITM1 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:15 kDa)
NCBI and Uniprot Product Information
Uniprot Description
IFITM1: IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV). Can inhibit: influenza virus hemagglutinin protein- mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation. Belongs to the CD225/Dispanin family.
Protein type: Cell cycle regulation; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: membrane; integral to membrane; plasma membrane
Molecular Function: protein binding; receptor signaling protein activity
Biological Process: positive regulation of osteoblast differentiation; negative regulation of cell proliferation; regulation of immune response; ossification; cell surface receptor linked signal transduction; negative regulation of viral genome replication; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; response to virus; defense response to virus; negative regulation of cell migration
Research Articles on IFITM1
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Product Notes
The IFITM1 ifitm1 (Catalog #AAA8100334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IFITM1/CD225 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IFITM1/CD225 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC-P: 1:10,000-1:50,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the IFITM1 ifitm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFITM1/CD225, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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