Rabbit anti-Human, Mouse IDH Polyclonal Antibody | anti-IDH antibody

Isocitrate Dehydrogenase [NADP], Cytoplasmic (IDH, IDCD, IDH1, IDP, IDPC, Cytosolic NADP-isocitrate Dehydrogenase, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD) (MaxLight 490)

Cat. Num. AAA6483640

• Gene Names: IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: IDH; Polyclonal Antibody; Isocitrate Dehydrogenase [NADP]; Cytoplasmic (IDH; IDCD; IDH1; IDP; IDPC; Cytosolic NADP-isocitrate Dehydrogenase; NADP(+)-specific ICDH; Oxalosuccinate Decarboxylase; PICD) (MaxLight 490); Isocitrate Dehydrogenase 1 (NADP+); Soluble; EC=1.1.1.42; anti-IDH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IDH1 at ~47kD. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-IDH antibody: Western Blot (WB), FLISA, Immunohistochemistry (IHC)
• Application Notes: WB: 1:50-1:100; FLISA: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa122-152 from the center region of human IDH1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of IDH1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of IDH1 Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-IDH antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Geisbrecht B.V., Gould S.J.J. Biol. Chem. 274:30527-30533(1999). 2. Xu X., Zhao J., Xu Z.J. Biol. Chem. 279:33946-33957(2004). 3. Bleeker F.E., Lamba S.Hum. Mutat. 30:7-11(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 28178825
• NCBI GeneID: 3417
• NCBI Accession #: NP_005887
• NCBI GenBank Nucleotide #: NM_005896.3
• UniProt Accession #: O75874; Q567U4; Q6FHQ6; Q7Z3V0; Q93090; Q9NTJ9; Q9UKW8
• Molecular Weight: 46,659 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 1 (NADP+), soluble
• NCBI Official Symbol: IDH1
• NCBI Official Synonym Symbols: IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
• NCBI Protein Information: isocitrate dehydrogenase [NADP] cytoplasmic; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; oxalosuccinate decarboxylase; epididymis luminal protein 216; epididymis secretory protein Li 26; NADP-dependent isocitrate dehydrogenase, cyt
• UniProt Protein Name: Isocitrate dehydrogenase [NADP] cytoplasmic
• UniProt Gene Name: IDH1
• UniProt Synonym Gene Names: PICD; IDH
• UniProt Entry Name: IDHC_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

IDH1: an oxidoreductase that catalyzes the third step of the TCA cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH1 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. IDH1 is located in the cytosol and peroxisomes. Somatic mutations affecting arginine 132 (R132) are found in 80% of grade II?III gliomas and secondary glioblastomas in humans, and cause disease in a tissue-specific fashion. Only a single copy of the gene has been found to be mutated in tumours. Mutations of R132 to H, C, S, G, V, or L have been reported to be neomorphic, abolishing the conversion of isocitrate to alpha-KG. Instead, alpha-KG is converted to R(-)-2-hydroxyglutarate (2HG). Elevated levels of 2HG correlate with an elevated risk of malignant brain tumors. Neomorphic mutations in IDH1 and IDH2 convert alpha-KG to 2-hydroxyglutarate (2-HG) occur in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML).

Protein type: Other Amino Acids Metabolism - glutathione; Oxidoreductase; EC 1.1.1.42; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: peroxisomal matrix; mitochondrion; cytoplasm; peroxisome; cytosol

Molecular Function: protein homodimerization activity; magnesium ion binding; isocitrate dehydrogenase (NADP+) activity; NADP binding; NAD binding; receptor binding

Biological Process: isocitrate metabolic process; NADPH regeneration; glyoxylate cycle; glutathione metabolic process; response to steroid hormone stimulus; tricarboxylic acid cycle; response to oxidative stress; cellular lipid metabolic process; female gonad development; 2-oxoglutarate metabolic process

Disease: Glioma Susceptibility 1