Rabbit anti-Human, Mouse ID4 Polyclonal Antibody | anti-ID4 antibody

ID4, NT (ID4, BHLHB27, DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, Inhibitor of DNA binding 4) (PE)

Cat. Num. AAA6309155

• Gene Names: ID4; IDB4; bHLHb27
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ID4; Polyclonal Antibody; NT (ID4; BHLHB27; DNA-binding protein inhibitor ID-4; Class B basic helix-loop-helix protein 27; Inhibitor of DNA binding 4) (PE); Inhibitor of DNA binding 4; anti-ID4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ID4 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ID4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ID4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ID4 Antibody (N-term) in mouse bladder tissue lysates (35ug/lane). ID4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ID4 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ID4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ID4 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ID4 antibody

ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo-and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins

Product Categories/Family for anti-ID4 antibody

Antibodies; Binding Proteins

References

Fontemaggi, G., et al. Nat. Struct. Mol. Biol. 16(10):1086-1093(2009). Uhm, K.O., et al. J. Korean Med. Sci. 24(3):493-497(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4504573
• NCBI GeneID: 3400
• NCBI Accession #: NP_001537.1
• NCBI GenBank Nucleotide #: NM_001546.3
• UniProt Accession #: P47928; Q13005
• Molecular Weight: 16,622 Da
• NCBI Official Full Name: DNA-binding protein inhibitor ID-4
• NCBI Official Synonym Full Names: inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
• NCBI Official Symbol: ID4
• NCBI Official Synonym Symbols: IDB4; bHLHb27
• NCBI Protein Information: DNA-binding protein inhibitor ID-4; class B basic helix-loop-helix protein 27
• UniProt Protein Name: DNA-binding protein inhibitor ID-4
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: ID4
• UniProt Synonym Gene Names: BHLHB27; bHLHb27
• UniProt Entry Name: ID4_HUMAN

NCBI Description

This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011]

Uniprot Description

ID4: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; transcription corepressor activity

Biological Process: fat cell differentiation; circadian rhythm; transcription, DNA-dependent; myelination in the central nervous system; hippocampus development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; cerebral cortex neuron differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of oligodendrocyte differentiation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; positive regulation of cell proliferation; negative regulation of astrocyte differentiation; neuroblast proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle