Rabbit ID1 Polyclonal Antibody | anti-ID1 antibody

ID1 Polyclonal Antibody

Cat. Num. AAA3006336

• Gene Names: ID1; ID; bHLHb24
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ID1; Polyclonal Antibody; ID1 Polyclonal Antibody; DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; bHLHb24; Inhibitor of DNA binding 1; BHLHB24; ID; anti-ID1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ID1 polyclonal antibody detects endogenous levels of ID1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 155

• Applicable Applications for anti-ID1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human ID1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ID1 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Mouse liver tissue lysateLane3:Rat iver tissue lysate)

Related Product Information for anti-ID1 antibody

Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

NCBI and Uniprot Product Information

• NCBI GI #: 31317299
• NCBI GeneID: 3397
• NCBI Accession #: NP_002156.2
• NCBI GenBank Nucleotide #: NP_002156.2
• UniProt Accession #: P41134
• Molecular Weight: ~ 16kDa
• NCBI Official Full Name: DNA-binding protein inhibitor ID-1 isoform a
• NCBI Official Synonym Full Names: inhibitor of DNA binding 1, HLH protein
• NCBI Official Symbol: ID1
• NCBI Official Synonym Symbols: ID; bHLHb24
• NCBI Protein Information: DNA-binding protein inhibitor ID-1
• UniProt Protein Name: DNA-binding protein inhibitor ID-1
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: ID1
• UniProt Synonym Gene Names: BHLHB24; ID; bHLHb24
• UniProt Entry Name: ID1_HUMAN

NCBI Description

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ID1: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 20q11

Cellular Component: nucleoplasm; Golgi apparatus; nucleus

Molecular Function: protein dimerization activity; protein binding; transcription factor activity

Biological Process: circadian rhythm; collagen metabolic process; blood vessel endothelial cell migration; transcription, DNA-dependent; protein destabilization; heart development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; BMP signaling pathway; response to antibiotic; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; blood vessel morphogenesis; negative regulation of osteoblast differentiation; endothelial cell morphogenesis; angiogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis