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Western Blot (WB) (Western blot analysis of extracts from 3T3 cells, treated with PBS (10uM, 60mins), using ICK (Ab-159) antibody.)

Rabbit anti-Human, Mouse ICK Polyclonal Antibody | anti-ICK antibody

ICK (Ab-159) Antibody

Gene Names
ICK; ECO; MRK; LCK2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ICK; Polyclonal Antibody; ICK (Ab-159) Antibody; EC 2.7.11.22; hICK; Intestinal cell kinase; KIAA0936; anti-ICK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ICK protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
632
Applicable Applications for anti-ICK antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:3000
Immunogen Type
Peptide
Immunogen Description
Synthesized non-phosphopeptide derived from human ICK around the phosphorylation site of tyrosine 159 (T-D-Y(p)-V-S).
Target Name
ICK
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from 3T3 cells, treated with PBS (10uM, 60mins), using ICK (Ab-159) antibody.)

Western Blot (WB) (Western blot analysis of extracts from 3T3 cells, treated with PBS (10uM, 60mins), using ICK (Ab-159) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from 293 cells (Lane 2) and cos-7 cells (Lane 3), using ICK (Ab-159) antiobdy. The lane on the left is treated with synthesized peptide.)

Western Blot (WB) (Western blot analysis of extracts from 293 cells (Lane 2) and cos-7 cells (Lane 3), using ICK (Ab-159) antiobdy. The lane on the left is treated with synthesized peptide.)
Related Product Information for anti-ICK antibody
May play a key role in the development of multiple organ systems and particularly in cardiac development By similarity.Togawa K.,J. Cell. Physiol. 183:129-139(2000).Yang T.,Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.Nagase T.,DNA Res. 6:63-70(1999).
Product Categories/Family for anti-ICK antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,071 Da
NCBI Official Full Name
serine/threonine-protein kinase ICK
NCBI Official Synonym Full Names
intestinal cell (MAK-like) kinase
NCBI Official Symbol
ICK
NCBI Official Synonym Symbols
ECO; MRK; LCK2
NCBI Protein Information
serine/threonine-protein kinase ICK
UniProt Protein Name
Serine/threonine-protein kinase ICK
UniProt Gene Name
ICK
UniProt Synonym Gene Names
KIAA0936; hICK; LCK2; MRK
UniProt Entry Name
ICK_HUMAN

NCBI Description

Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ICK: a CMGC kinase of the RCK family. May play a role in cardiac development. Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Also expressed in many cancer cell lines. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; CMGC group; RCK family

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; cyclin-dependent protein kinase activity; magnesium ion binding; ATP binding

Biological Process: regulation of cell cycle; multicellular organismal development; intraflagellar transport; cilium biogenesis; signal transduction; protein amino acid phosphorylation

Disease: Endocrine-cerebroosteodysplasia

Research Articles on ICK

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Product Notes

The ICK ick (Catalog #AAA9409751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ICK (Ab-159) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ICK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the ICK ick for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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