Western Blot (WB)
(Western blot analysis of extracts from MCF7, using iASPP Antibody. The lane on the left was treated with blocking peptide.)
Rabbit iASPP Polyclonal Antibody | anti-PPP1R13L antibody
iASPP Antibody
Predicted Reactivity: Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using iASPP Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using iASPP Antibody. The lane on the left was treated with blocking peptide.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612191 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612191) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612191 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612191) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Subcellular Location: Cytoplasm. Nucleus. Note: Predominantly cytoplasmic but also nuclear.
Tissue Specificity: Highly expressed in heart, placenta and prostate. Weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte.
Subunit Structure: Interacts with RELA NF-kappa-B subunit and with SP1 via its C-terminus part. Interacts (via SH3 domain and ANK repeats) with p53/TP53; the interaction inhibits pro-apoptotic activity of p53/TP53 (PubMed:12524540). Interacts with TP63 and TP73.
Similarity: The N-terminal region is required for cytoplasmic localization.The ANK repeats and the SH3 domain are required for specific interactions with p53/TP53.Belongs to the iASPP family.
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)89kDa.
NCBI Description
IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1 (MIM 606455) and ASPP2 (MIM 602143) are activators of p53.[supplied by OMIM, Mar 2008]
Uniprot Description
PPP1R13L: an oncoprotein that interacts with and inhibits NF-kappaB, Sp1 and p53. Plays a central role in regulation of apoptosis and transcription. May inhibit p53 by preventing its association with ASPP1 or ASPP2, thereby suppressing the subsequent activation of apoptosis. Required for neuronal survival after axonal injury. Two alternatively spliced isoforms have been described. Isoform 1 is preferrentially found in the cytoplasm; isoform 2 is nuclear.
Protein type: Phosphatase, regulatory subunit; Apoptosis; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: intercellular bridge; cytoplasm; cell junction; nucleus
Molecular Function: identical protein binding; protein binding; transcription corepressor activity; transcription factor binding
Biological Process: transcription, DNA-dependent; apoptosis; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; multicellular organismal homeostasis; embryonic camera-type eye development; post-embryonic development; cardiac muscle contraction; hair cycle
Research Articles on PPP1R13L
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Product Notes
The PPP1R13L ppp1r13l (Catalog #AAA9612191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The iASPP Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's iASPP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:500 ICC: 1:100-1:500 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PPP1R13L ppp1r13l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "iASPP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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