Western Blot (WB)
(Western Blot analysis of extracts of various cells, using IARS antibody.)
Rabbit IARS Polyclonal Antibody | anti-IARS antibody
IARS Polyclonal Antibody
Gene Names
IARS1; IRS; IARS; ILRS; ILERS; GRIDHH; PRO0785
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
IARS; Polyclonal Antibody; IARS Polyclonal Antibody; Isoleucine--tRNA ligase; cytoplasmic; Isoleucyl-tRNA synthetasel; IleRS; anti-IARS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
IARS polyclonal antibody detects endogenous levels of IARS protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1262
Applicable Applications for anti-IARS antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
Recombinant protein of human IARS.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using IARS antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using IARS antibody.)
Related Product Information for anti-IARS antibody
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 144kDa
NCBI Official Full Name
isoleucine--tRNA ligase, cytoplasmic isoform 1
NCBI Official Synonym Full Names
isoleucyl-tRNA synthetase 1
NCBI Official Symbol
IARS1
NCBI Official Synonym Symbols
IRS; IARS; ILRS; ILERS; GRIDHH; PRO0785
NCBI Protein Information
isoleucine--tRNA ligase, cytoplasmic
UniProt Protein Name
Isoleucine--tRNA ligase, cytoplasmic
UniProt Gene Name
IARS
UniProt Synonym Gene Names
IRS; IleRS
NCBI Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012]
Research Articles on IARS
Similar Products
Product Notes
The IARS iars (Catalog #AAA3008432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IARS Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IARS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the IARS iars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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