Rabbit anti-Human I kappa B-alpha Polyclonal Antibody | anti-I kappa B-alpha antibody

I kappa B-alpha antibody

Cat. Num. AAA9404605

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: I kappa B-alpha; Polyclonal Antibody; I kappa B-alpha antibody; anti-I kappa B-alpha antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 317

• Applicable Applications for anti-I kappa B-alpha antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunofluorescence: 1:100-1:200
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 305 of Human NFKBIA
• Target Name: I kappa B-alpha
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE Primary antibody diluted at 1: 1000)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha antibody at 1: 200 dilution.)

Related Product Information for anti-I kappa B-alpha antibody

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Product Categories/Family for anti-I kappa B-alpha antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390
• NCBI GenBank Nucleotide #: NM_020529.2
• Molecular Weight: 35,609 Da
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
• UniProt Entry Name: IKBA_HUMAN

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

IkB-alpha: a regulatory protein that inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation.

Protein type: Inhibitor; DNA-binding

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: cytoplasm; cytosol; nucleus; plasma membrane

Molecular Function: enzyme binding; heat shock protein binding; identical protein binding; NF-kappaB binding; nuclear localization sequence binding; protein binding; protein complex binding; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; apoptosis; cytoplasmic sequestering of NF-kappaB; cytoplasmic sequestering of transcription factor; inhibition of NF-kappaB transcription factor; innate immune response; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of DNA binding; negative regulation of myeloid cell differentiation; negative regulation of Notch signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of cellular protein metabolic process; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; protein import into nucleus, translocation; regulation of cell proliferation; regulation of NF-kappaB import into nucleus; response to exogenous dsRNA; response to muramyl dipeptide; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; viral reproduction

Disease: Ectodermal Dysplasia, Anhidrotic, With T-cell Immunodeficiency, Autosomal Dominant