Rabbit anti-Human HYAL2 Polyclonal Antibody | anti-HYAL2 antibody

HYAL2, ID (HYAL2, LUCA2, Hyaluronidase-2, Hyaluronoglucosaminidase-2, Lung carcinoma protein 2) (AP)

Cat. Num. AAA6308969

• Gene Names: HYAL2; LUCA2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HYAL2; Polyclonal Antibody; ID (HYAL2; LUCA2; Hyaluronidase-2; Hyaluronoglucosaminidase-2; Lung carcinoma protein 2) (AP); Lung carcinoma protein 2; anti-HYAL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HYAL2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HYAL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-157 amino acids from the Central region of human HYAL2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HYAL2 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. HYAL2 Antibody (Center) (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(HYAL2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HYAL2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-HYAL2 antibody

This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.

Product Categories/Family for anti-HYAL2 antibody

Antibodies; Cancer Markers

References

Monzon, M.E., et al. J. Biol. Chem. 285(34):26126-26134(2010). Tzuman, Y.C., et al. Neoplasia 12(1):51-60(2010). de la Motte, C., et al. Am. J. Pathol. 174(6):2254-2264(2009). Nykopp, T.K., et al. BMC Cancer 9, 143 (2009): Miller, A.D., et al. Osteoarthr. Cartil. 14(12):1315-1317(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 15022801
• NCBI GeneID: 8692
• NCBI Accession #: NP_003764.3; NP_149348.2
• NCBI GenBank Nucleotide #: NM_003773.4
• UniProt Accession #: Q12891; O15177; Q9BW29; B3KRZ2
• Molecular Weight: 53,860 Da
• NCBI Official Full Name: hyaluronidase-2
• NCBI Official Synonym Full Names: hyaluronoglucosaminidase 2
• NCBI Official Symbol: HYAL2
• NCBI Official Synonym Symbols: LUCA2
• NCBI Protein Information: hyaluronidase-2; hyal-2; PH20 homolog; PH-20 homolog; hyaluronidase 2; lysosomal hyaluronidase; lung carcinoma protein 2; hyaluronoglucosaminidase-2
• UniProt Protein Name: Hyaluronidase-2
• UniProt Gene Name: HYAL2
• UniProt Synonym Gene Names: LUCA2; Hyal-2; LuCa-2

NCBI Description

This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.[provided by RefSeq, Mar 2010]

Uniprot Description

Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.