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Immunofluorescence (IF) (Immunofluorescence staining of ELAVL1 in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ELAVL1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to nucleus.)

Rabbit HuR/ELAVL1 Polyclonal Antibody | anti-ELAVL1 antibody

Anti-HuR/ELAVL1 Antibody, Rabbit Polyclonal

Gene Names
ELAVL1; HUR; Hua; MelG; ELAV1
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
HuR/ELAVL1; Polyclonal Antibody; Anti-HuR/ELAVL1 Antibody; Rabbit Polyclonal; ELAVL1 Antibody; Rabbit PAb; Antigen Affinity Purified; ELAV like RNA binding protein 1; Anti-ELAV1 Antibody; Anti-Hua Antibody; Anti-HUR Antibody; Anti-MelG Antibody; anti-ELAVL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human HuR/ELAVL1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ELAVL1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ICC/IF: 1:1500-1:50,000
IP: 0.2-1uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human ELAVL1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ELAVL1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of ELAVL1 in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ELAVL1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to nucleus.)

Immunofluorescence (IF) (Immunofluorescence staining of ELAVL1 in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ELAVL1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to nucleus.)

Immunoprecipitation (IP)

(ELAVL1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-ELAVL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-ELAVL1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa)

Immunoprecipitation (IP) (ELAVL1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-ELAVL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-ELAVL1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa)

Western Blot (WB)

(Anti-ELAVL1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa)

Western Blot (WB) (Anti-ELAVL1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa)
Related Product Information for anti-ELAVL1 antibody
HuR/Elav-like RNA binding protein 1 (ELAVL1) positively regulates mRNA stability of AU-rich elements (ARE)-containing transcript such as pro-inflammatory cytokines. Ribotoxic stresses can trigger the production of pro-inflammatory mediators by enhancing mRNA stability and transcriptional activity. We investigated the effects of ribotoxin deoxynivalenol (DON) on HuR translocation and its involvement in the regulation of the pro-inflammatory interleukin-8 (IL-8) mR. ELAVL1 is an RNA binding protein involved in translation control, which might have a regulatory role in prostate cancer progress. ELAVL1 is an RNA binding protein involved in translation control, which might have a regulatory role in prostate cancer progress. EIF5A2, eukaryotic translation initiation factor 5A2, is associated with several human cancers. Overexpression of EIF5A2 was an independent predictor for poor metastasis-free survival of localized invasive BC patients treated with radical cystectomy. eIF5A2 is overexpressed by gene amplification or hypoxia in ESCCs, and associated with up-regulation of HIF1alpha, metastasis, and shorter survival times of patients. Increased expression of eIF5A2 increases metastasis and angiogenesis in ESCC via the HIF1alpha-mediated signaling pathway.
Product Categories/Family for anti-ELAVL1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
326
NCBI Official Full Name
ELAV-like protein 1
NCBI Official Synonym Full Names
ELAV like RNA binding protein 1
NCBI Official Symbol
ELAVL1
NCBI Official Synonym Symbols
HUR; Hua; MelG; ELAV1
NCBI Protein Information
ELAV-like protein 1; Hu antigen R; hu-antigen R; embryonic lethal, abnormal vision, drosophila, homolog-like 1; ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
UniProt Protein Name
ELAV-like protein 1
Protein Family
UniProt Gene Name
ELAVL1
UniProt Synonym Gene Names
HUR; HuR
UniProt Entry Name
ELAV1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]

Uniprot Description

HuR: Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ubiquitous. Belongs to the RRM elav family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function: mRNA binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding

Biological Process: positive regulation of translation; multicellular organismal development; mRNA stabilization; gene expression

Research Articles on ELAVL1

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Product Notes

The ELAVL1 elavl1 (Catalog #AAA8100787) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HuR/ELAVL1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's HuR/ELAVL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:1000 ICC/IF: 1:1500-1:50,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the ELAVL1 elavl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HuR/ELAVL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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